贵州医科大学学报

2008, No.135(06) 678-680

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

环维黄杨星D对Na_2S_2O_4诱导EVC304细胞损伤的保护作用
Effects of Cyclovirobuxine D Protecting EVC304 Cell from Injury Induced by Na_2S_2O_4 in vitro

袁冬平;许立;龙军;梁涛;陶玲;方泰惠;
YUAN Dongping2,XU Li1,LONG Jun1,LIANG Tao1,TAO Ling2,FANG Taihui1 (1. Department of Pharmacology,Nanjing University of Traditional Chenise Medicine,Nanjing 210046,Jiangsu,China; 2. School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:研究环维黄杨星D(CVB-D)对Na2S2O4诱导EVC304细胞损伤的保护作用。方法:采用体外培养人脐静脉内皮细胞EVC304,Na2S2O4作为耗氧剂建立细胞缺氧损伤模型。用MTT法测定细胞存活率与细胞培养液乳酸脱氢酶(LDH)活力反映细胞损伤的程度,培养液一氧化氮合酶(NOS)活力与内皮素(ET)含量分析EVC304细胞的功能。结果:CVB-D能显著抑制Na2S2O4诱导EVC304细胞OD570的降低和细胞培养液中LDH活力的增加,提高培养液中NOS活性,降低ET的含量(与模型组比较,P<0.05或P<0.01)。结论:CVB-D对Na2S2O4诱导EVC304细胞损伤具有保护作用,其作用机制可能与改善NOS和ET失调有关。
Objective:To investigate the protective effects of cyclovirobuxine D (CVB-D)on human umbilical vein endothelial cell (EVC304) from the injury induced by Na2S2O4 in vitro. Methods:Cell injury model of cultured EVC304 was induced by Na2S2O4 as oxygen comsuming agent. Cell injury degree was determined with methyl thiazolyl tetrazolium (MTT) stainning method.and lactate dehydrogenase (LDH) activity in culturing medium. Cellular function of EVC304 was indicated by nitric oxide syntyhase (NOS) activity and endothelin (ET) content in the medium. Results:Cell survival ratio and NOS activity significantly decreased after treated with Na2S2O4,and the LDH activity and ET concents significantly increased (P<0.05,P<0.01). CVB-D remarkablely attenuated EVC304 injury induced by Na2S2O4. Conclusions:CVB-D can protect ECV304 from injury induced by Na2S2O4. The protective mechanism may relate to ameliorating disturbance of NOS and ET of ECV304.

关键词(KeyWords): 环维黄杨星-D;内皮,血管;脐静脉;乳酸脱氢酶;内皮素;一氧化氮合酶
cyclovirobuxine D; endothelium,vascular; umbilical veins; lactate dehydrogenase; nitric-oxide syntyhase; endothelin

Abstract:

Keywords:

基金项目(Foundation): 贵州省高层次人才特助经费资助项目[TZJF-2006-13]

作者(Author): 袁冬平;许立;龙军;梁涛;陶玲;方泰惠;
YUAN Dongping2,XU Li1,LONG Jun1,LIANG Tao1,TAO Ling2,FANG Taihui1 (1. Department of Pharmacology,Nanjing University of Traditional Chenise Medicine,Nanjing 210046,Jiangsu,China; 2. School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2008.06.031

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享