致倦库蚊发育各期卵、幼虫及蛹匀浆中的游离氨基酸Analysis of Free Amino Acids in Homogenate of the Eggs,Larvae and Pupas of Culex Pipiens Quinqefasciatus
李建华,魏明,王菊生,包怀恩
LI Jian hua; WEI Ming; WANG Ju sheng; BAO Huai en (Department of Parasitology;
摘要(Abstract)：
目的 :探讨致倦库蚊不同发育期虫体匀浆游离氨基酸的动态。方法 :应用高效液相色谱仪对致倦库蚊的虫卵和Ⅰ、Ⅱ、Ⅲ、Ⅳ期幼虫及雌、雄蛹等虫体匀浆中的游离氨基酸进行检测。结果 :1 虫卵随发育时间的不同 ,其氨基酸的种类发生明显变化 ,色氨酸在发育 8h后才出现 ,谷氨酸逐渐减少 ,而蛋氨酸、赖氨酸等逐渐增多 ,但总量变化不显著。 2 幼虫随着虫龄的增加 ,虫体氨基酸含量明显增多 ,Ⅳ期幼虫的氨基酸含量明显高于其他各期幼虫。蛋氨酸含量最高 ,鸟氨酸、天门冬氨酸等次之。天门冬氨酸、谷氨酸、甘氨酸和苏氨酸、精氨酸等在Ⅳ期幼虫体内的氨基酸含量达到最高峰。 3 蛹体内的氨基酸总量与蚊蛹性别关系不明显 ,但在雌、雄蛹间天门冬氨酸、苯丙氨酸、鸟氨酸、组胺酸等氨基酸的含量却有显著性差异。结论 :不同发育期致倦库蚊匀浆内游离氨基酸的种类及含量均有差异
Objective:To investigate the change of the free amino acids (FAAs) in the developing stage of Culex pipiens quinqefaciatus.Methods: FAAs in homogenate of the eggs,larvae(Ⅰ,Ⅱ,Ⅲ and Ⅳ instar) and pupas (female and male) of Culex pipiens quinqefaciatus were detected respectively by High Perfomance Liquid Chromatography(LC-3A).Results:The results showed that the contents and species of FAAs changed with the development stages of mosquitoes respectively.①GLU reduced and MET,LYS increased significantly, but the total change of FAAs was not remarkable in the development eggs.TRY was detected in the eggs at the 8th four after development.②With the larvae development,the totae FAAs was increased remarkably,especially in the 4th instar larvae.The quantity of MET was the highest in 20 kinds of amino acids.ASP,GLU,G+T and ARG which got the maximum level at the 4th instar larvae.③ The total FAAs was not remarkable difference between the female and male mosquito pupas.The contents of ASP,PHE,ORN,HIS in the female pupas were higher than those in the males. Conclusion:The contents and species of FAAs have changed significantly at different developing stages of mosguitoes.
关键词(KeyWords)：
库蚊属;卵;幼虫;蛹;氨基酸类;致倦库蚊
culex;ovum;larva; pupa;amino acids; Cules pipiens quinqefaciatus
基金项目(Foundation): 贵州省卫生厅资助课题
作者(Authors):
李建华,魏明,王菊生,包怀恩
LI Jian hua; WEI Ming; WANG Ju sheng; BAO Huai en (Department of Parasitology;
DOI: 10.19367/j.cnki.1000-2707.2001.01.002
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