武雅楠,文赛,梁秋果,杨茜,吴家红,朱儒芳,程金芝
WU Yanan,WEN Sai,LIANG Qiuguo,YANG Xi,WU Jiahong,ZHU Rufang,CHENG Jinzhi

• 1:贵州医科大学现代病原生物学实验室
• 2:贵州医科大学寄生虫学教研室
• 3:贵阳市第二实验中学

摘要(Abstract)：

目的:了解贵阳市常见蚊虫种群中沃尔巴克体(Wolbachia)的感染情况及基因型。方法:采用勺舀法采集贵阳市6城区(云岩区、南明区、花溪区、修文县、息烽县和开阳县)的蚊虫并鉴定;选用Wolbachia表面蛋白基因(wsp),利用PCR方法对单只雌蚊进行扩增、测序并做系统发育分析。结果:在贵阳市6城区不同水体采集到的常见蚊种有3种,分别是白纹伊蚊、致倦库蚊和骚扰阿蚊,3种蚊虫种群Wolbachia的核酸阳性率为42.9%~95.0%;获得13条目的基因序列、序列长度为572~602 bp,系统发育树显示这些序列分别属于A和B两个超群,白纹伊蚊Wolbachia基因型为w Alb A和w Alb B,致倦库蚊Wolbachia基因型为w Pip,骚扰阿蚊Wolbachia基因型为w Alb A。结论:贵阳市白纹伊蚊、致倦库蚊和骚扰阿蚊普遍感染Wolbachia,其基因型为wAlb A、wAlb B和wPip。
Objective: To investigate the infection status and genotype of symbiotic bacteria( Wolbachia) in common mosquito in Guiyang. Methods: Mosquitoes were collected by using a spoon from 6 districts of Guiyang( Yunyan,Nanming,Huaxi,Xiuwen,Xifeng and Kaiyang) and identified by morphology in the lab. Wolbachia surface protein gene( wsp) was amplified by PCR and sequenced for phylogenetic analysis. Results: There were 3 species mosquito found in the different habitats in the 6 districts,Aedes albopictus,Culex pipens pallens and Armigeres obturbans. The positive rate of wsp ranged from 42. 9% to 95%. 13 target gene sequences were obtained with sequence length between572 to 602 bp. Based on the phylogenetic tree,these sequences were divided into A and B supergroup respectively. The wolbachia genotype of A albopictus was w Alb A and w Alb B. The Wolbachia genotype of Culex pipens pallens was w Pip. The Wolbachia genotype of Armigeres obturbans was w Alb A. Conclusions: Aedes albopictus,Culex pipens pallens and Armigeres obturbans in Guiyang are generally infected with Wolbachia and their genotypes are wAlb A,wAlb B and wPip.

关键词(KeyWords)： 沃尔巴克氏体;wsp基因;白纹伊蚊;致倦库蚊;骚扰阿蚊
Wolbachia;wsp gene;Aedes albopictus;Culex pipens pallens;Armigeres obturbans

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81260260);; 贵州省科学技术厅基金平台建设项目[黔科平台(2012)4006];; 贵州省大学生创新创业训练项目(201610660033)

作者(Author): 武雅楠,文赛,梁秋果,杨茜,吴家红,朱儒芳,程金芝
WU Yanan,WEN Sai,LIANG Qiuguo,YANG Xi,WU Jiahong,ZHU Rufang,CHENG Jinzhi

DOI: 10.19367/j.cnki.1000-2707.2017.10.004

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