刘华,冯光霞,雷霆雯,李蓉,吴宁
LIU Hua,FENG Guangxia,LEI Tingwen,LI Rong,WU Ning

• 1:贵州医科大学生物化学与分子生物学教研室
• 2:贵州医科大学口腔医学院
• 3:贵州医科大学化学教研室
• 4:贵州医科大学化学与生物化学实验室

摘要(Abstract)：

目的:探讨花楸猕猴桃根对肝细胞CYP1A1酶的作用机制。方法:40正常SD大鼠随机分为空白对照组和猕猴桃根提取物高、中、低剂量组(50 g/kg、25 g/kg、13 g/kg),连续灌胃7 d,2次/d,处死大鼠后取出肝脏,通过检测N-脱甲基酶活性反映细胞CYP1A1酶活性,提取组织RNA逆转录后用RT-PCR检测CYP1A1mRNA表达水平。结果:与空白对照组相比,花楸猕猴桃根高、中、低剂量组CYP1A1酶活性均明显受到抑制,且呈浓度依赖性(P<0.05),但各剂量组间无显著性差异;与空白对照组比较,花楸猕猴桃根高、中、低药物剂量组能显著抑制CYP1A1 mRNA的表达(P<0.05)。结论:花楸猕猴桃根可下调大鼠肝细胞CYP1A1酶的活性,并抑制其mRNA表达水平,这可能是花楸猕猴桃根能够预防癌症的作用机制。
Objective: To explore the effects of roots of Actinidia sorbifolia C. F. Liang on CYP1A1 in hepatocyte of rats and the mechanism. Methods: The 40 Wistar rats were randomly divided into 4groups:blank control group,high dose of root group,middle dose of root group and low dose of root group. The rats received intragastric administration of water decoction of roots of Actinidia sorbifolia C.F. Liang( high dose group 50 g / kg,middle dose group 25 g / kg,low dose group 13 g / kg,repectively.) continuously for 7 days,twice daily. The rats were killed and liver tissue was taken to detect the enzymatic activity of CYP1A1 by detecting activity of ADM,electrophoresis method was adopted to check the integrity of extracted RNA sample,and RT-PCR was used to detect the mRNA expression level of CYP1A1. Results: The experimental results showed that compared with blank group,the enzymatic activity of CYP1A1 in high,middle,and low dose group was significantly inhibited in a dosage dependant manner( P < 0. 05),but there was no significant differences between high,middle and low dose group. Besides,the mRNA expression levels of CYP2A1 were inhibited in high,middle,and low dose group( P < 0. 05). Conclusion: The roots of Actinidia sorbifolia C. F. Liang can down-regulate the enzymatic activity of CYP1A1 and inhibit the mRNA expression level of CYP1A1,which is possibly related to its prevention of cancer.

关键词(KeyWords)： 花楸猕猴桃根;细胞色素P450 CYP1A1;逆转录聚合酶链反应;大鼠,Sprague-Dawley
roots of Actinidia sorbifolia C.F.Liang;cytochrome P450 CYP1A1;reverse transcription polymerase chain reaction;rats,Sprague-Dawley

Abstract:

Keywords:

基金项目(Foundation): 贵阳市科学技术计划项目(2006筑科农第39号)

作者(Author): 刘华,冯光霞,雷霆雯,李蓉,吴宁
LIU Hua,FENG Guangxia,LEI Tingwen,LI Rong,WU Ning

DOI: 10.19367/j.cnki.1000-2707.2015.08.011

参考文献(References)：

• [1]林国彪,钟振国,张雯艳.山梨猕猴桃根提取物抗肿瘤作用的机制研究[J].中国中药杂志,2008(16):2011-2014.
• [2]陈琳,阮昊,姚彤炜.地非三唑对大鼠肝细胞细胞色素P450 CYPl Al/2的诱导作用[J].中国药理学与毒理学杂志,2007(6):501-504.
• [3]Mrozikiewicz PM,Grzeskowiak E,Seremak-Mrozikiewicz A,et a1.Importance of CYPl Al polymorphism and its transcriptional regulation in ovarian and endometrial cancer[J].Ginekol Pol,2011(12):925-932.
• [4]黄凯,贺晴.CYPIAI基因多态性与子宫内膜癌相关性的研究进展[J].国际药学研究,2013(3):277-280.
• [5]张均田.现代药理实验学方法(下册)[M].北京医科大学中国协和医科大学联合出版社,1998:649.
• [6]Ahn T,Yun CH.Molecular mechanisms regulating the mitochondrial targeting of microsomal cytochrome P450 enzymes[J].Curr Drug Metab,2010(10):830-839.
• [7]钟强,郑智,邵姣梅,等.细胞色素P450ZJZ基因启动子区单核苷酸多态性与高血压及脑卒中的相关性[J].华中科技大学学报:医学版,2006(4):473-475.
• [8]Ma Q,Lu AY.CYPl A induction and human risk assessment:all evolving tale of in vitro and in vivo studies[J].Drag Metab Dispos,2007(7):1009-1016.
• [9]朱燕萍,金念祖.细胞色素P450 1 A在肿瘤化学预防方面的作用[J].国外医学卫生学分册,2003(30):227-230.

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