刘继刚,袁杨,臧蔚,刘昊
LIU Jigang,YUAN Yang,ZANG Yu,LIU Hao

• 1:遵化市人民医院口腔科
• 2:华北理工大学附属医院神经内科

摘要(Abstract)：

目的:探讨柴胡疏肝散对抑郁大鼠海马组织微管相关蛋白-2(MAP-2)磷酸化的影响。方法:成年雄性Wistar大鼠45只,随机均分为对照组、模型组和治疗组;模型组和治疗组采用慢性不可预性温和应激建立抑郁症大鼠模型,治疗组同时经灌胃给予柴胡疏肝散治疗,持续22 d;采用糖水实验、高架十字迷宫实验和Morris水迷宫实验观察各组大鼠行为学变化,Western blot方法检测各组大鼠海马组织MAP-2及磷酸化MAP-2(p MAP-2)表达,Real-time PCR检测p MAP-2 mRNA表达。结果:与对照组比较,模型组大鼠糖水偏好百分比明显降低,进入开臂次数和停留时间百分比增高,水迷宫逃避潜伏期延长,海马p MAP-2蛋白和mRNA表达水平明显降低,差异均有统计学意义(P<0.05);与模型组比较,治疗组大鼠糖水偏好百分比明显增高,进入开臂次数和停留时间百分比降低,水迷宫逃避潜伏期减少,海马p MAP-2蛋白和mRNA表达水平明显增高,差异均有统计学意义(P<0.05)。结论:柴胡疏肝散具有显著的抗抑郁作用,该作用可能与影响海马MAP-2磷酸化有关。
Objective: To observe the influence of bupleurum liver-coursing powder on phosphorylation of microtubule-associated protein 2( p MAP-2) in hippocampus of depressive rats. Methods: Forty-five male Wistar rats were randomly divided into control group,model group and treatment group.The depression animal model was produced by giving the rats chronic unpredicted mild stress and treatment group received intragastric administration of bupleurum liver-coursing powder for 22 days. The sucrose preference test,elevated plus maze test and Morris water maze test were adopted to observe behavioral changes of rats in each group. The expression of p MAP-2 protein and mRNA were detected by western blot and real-time PCR respectively. Results: Compared with control group,sucrose preference percentage of model group was significantly lower,the number of entry into open arms and percentage of detention time extended significantly,the escape latency of Morris water maze test increased significantly,and the expression of p MAP-2 protein and mRNA were decreased significantly( P <0. 05). Compared with model group,sucrose preference percentage of treatment was significantly higher,the number of entry into open arms and percentage of detention time decreased significantly,the escape latency of Morris water maze test decreased significantly,and the expression of p MAP-2 protein and mRNA were increased significantly( P < 0. 05). Conclusion: Bupleurum liver-coursing powder has a significant anti-depressant effect,and its mechanism may be relavent to its effect on the phosphorylation of MAP-2 in hippocampus.

关键词(KeyWords)： 柴胡疏肝散;抑郁症;海马;微管相关蛋白;大鼠,wistar
bupleurum liver-coursing powder;depression;hippocampus;microtubule-associated protein;rats,wistar

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81201048)

作者(Author): 刘继刚,袁杨,臧蔚,刘昊
LIU Jigang,YUAN Yang,ZANG Yu,LIU Hao

DOI: 10.19367/j.cnki.1000-2707.2016.04.006

参考文献(References)：

• [1]谭川雪,王飞飞,陈林木,等.抑郁症大鼠海马CA1区及齿状回毛细血管的体视学研究[J].重庆医科大学学报,2015(1):6-7.
• [2]隋竹欣,李珍,刘昊,等.抑郁大鼠海马微管蛋白p MAP-2和p-Tau表达的研究[J].神经解剖学杂志,2015(2):175-180.
• [3]周庆莹,刘宏岩.中医治疗抑郁症研究[J].长春中医药大学学报,2013(1)88-89.
• [4]刘英,徐爱军,田艳霞,等.柴胡舒肝散对抑郁症大鼠海马5-HT及5-HT1A表达的影响[J].河北北方学院学报,2013(5):65-67,70.
• [5]冯启明,罗红叶,韦波,等.广西壮族自治区农村居民抑郁症流行病学调查[J].中国公共卫生,2013(11):1610-1613.
• [6]王联庆,张锐,李长风.柴胡疏肝散治验四则[J].齐鲁医学杂志,2012(5):466-467.
• [7]王任昌,朱自强.柴胡疏肝散治疗抑郁症临床观察[J].实用中医药杂志,2013(4):258-259.
• [8]白钰,陈永灿.柴胡疏肝散治验3则[J].新中医,2013(7):212-213.
• [9]张黎明.抗抑郁剂调控海马细胞骨架的功能研究[D].北京:中国人民解放军军事医学科学院,2008.
• [10]Shucard JL,Cox J,Shucard DW,et al.Symptoms of posttraumatic stress disorder and exposure to traumatic stressors are related to brain structural volumes and behavioral measures of affective stimulus processing in police officers[J].Psychiatry Res,2012(1):25-31.
• [11]Fujii T,Saito DN,Yanaka HT,et al.Depressive mood modulates the anterior lateral CA1 and DG/CA3 during a pattern separation task in cognitively intact individuals:a functional MRI study[J].Hippocampus,2014(2):214-224.
• [12]Cobb JA,Simpson J,Mahajan CJ,et al.Hippocampal volume and total cell numbers in major depressive disorder[J].J Psychiatr Res,2013(3):299-306.

文章评论(Comment)：