何燕,单可人,吴昌学,谢渊,李毅,官志忠,任锡麟
HE Yan,SHAN Ke-ren,WU Chang-xue,XIE Yuan,LI Yi,GUAN Zhi-zhong,REN Xi-lin(The Key Laboratory of Molecular Biology

• 1:贵州省医学分子生物学重点实验室
• 2:贵州省医学分子生物学重点实验室
• 3:贵州省医学分子生物学重点实验室
• 4:贵州省医学分子生物学重点实验室

摘要(Abstract)：

目的:调查Y染色体短串联重复序列(short tandem repeat,STR)DYS388基因座及Y染色体特异的Alu序列插入基因座DYS287的遗传多态在贵州省三都水族群体中的多态性分布。方法:采用聚合酶链反应及荧光标记基因扫描技术,分别对DYS287和DYS388基因座的遗传多态性进行分析。结果:在94名无关水族男性个体中,DYS287全部显示为YAP-,DYS388基因座共检出4种等位基因,DYS388×12基因频率高达0.9681。结论:获得贵州三都水族群体DYS287和DYS388基因座的遗传学数据,为该民族遗传关系的分析、法医学鉴定及该民族的起源提供了一定的遗传背景资料。
Objective:To investigate the genetic polymorphism of chromosome Y specific short tandem repeat(Y-specific STR)locus DYS388 and the Y-chromosome specific Alu insert sequence DYS287 of Shui population in Shandu,Guizhou,China.Methods:DYS287 were amplified by PCR with special primers.DYS388 were amplified with fluorescently labeled primers,and genotypes were determined by ABI 377 Genetic Analyzer.Results:Among 94 unrelated males,4 alleles were observed in locus DYS388.The allele frequency of DYS388×12 was 0.968 1.In all samples,YAP element was absent.Conclusion:The genetic data of DYS287 and DYS388 loci in Shui population have been obtained,and they might be useful in studies of population origin,relationship between populations,and forensic applications.

关键词(KeyWords)： 基因频率;多态现象(遗传学);Y染色体;聚合酶链反应;少数民族;贵州;短串联重复序列
gene frequency;polymorphism(genetics);Y chromosome;polymerase chain reaction;minority groups;Guizhou;short tandem repeat

Abstract:

Keywords:

基金项目(Foundation): 贵州省优秀科技人才省长专项基金资助项目[黔科教办(2001)3];; 贵州省科技厅基金资助项目[黔科合(2004)3007]

作者(Author): 何燕,单可人,吴昌学,谢渊,李毅,官志忠,任锡麟
HE Yan,SHAN Ke-ren,WU Chang-xue,XIE Yuan,LI Yi,GUAN Zhi-zhong,REN Xi-lin(The Key Laboratory of Molecular Biology

DOI: 10.19367/j.cnki.1000-2707.2006.05.006

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