贵州医科大学学报

2011, v.36;No.149(02) 177-180

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

胃肠道间质瘤中TGF-β_1、uPA的表达及其意义
The Expression of TGF-β_1 and uPA in Gastrointestinal Stromal Tumors and Its Clinical Significance

赵滢;张天彪;王强;
ZHAO Ying1,ZHANG Tianbiao2,Wang Qiang1(1.Department of Gastro-intestinal Nutrition Surgery,the Affiliated Shengjing Hospital of China Medical University,Shenyang 110004,Liaoning,China;2.Department of Biochemistry and Molecular Biology,China Medical University,Shenyang 110001,Liaoning,China)

摘要(Abstract):

目的:探讨胃肠道间质瘤中TGF-β1、uPA的表达及其在胃肠道间质瘤(GIST)发生及发展中的作用。方法:应用RT-PCR和Western-blot方法检测93例GIST标本中TGF-β1、uPA mRNA和蛋白水平表达。结果:TGF-β1、uPA mRNA及蛋白在GIST组织中呈现高表达,TGF-β1、uPA mRNA及蛋白变化与NIH分级、肿瘤侵袭转移及黏膜受侵密切相关(P<0.05),二者呈现正相关(r=0.356,P<0.05)。结论:GIST中TGF-β1、uPA mRNA及蛋白均呈现高表达,并且可能在GIST发生发展中起重要作用。
Objective: To study the expression of TGF-β1 and uPA in gastrointestinal stromal tumors(GISTs),and to investigate their roles in genesis and development of GISTs.Methods: The expression of TGF-β1 and uPA mRNA and protein were detected with RT-PCR and western-blot in 93 cases of GISTs.Results: The expression of TGF-β1 and uPA mRNA and proteins was high in GISTs.The changes of TGF-β1 and uPA mRNA and proteins levels were closely associated with NIH grades,tumor metastasis,and invasion to mucosa(P<0.05).The TGF-β1 level positively correlated with uPA level(r=0.356,P<0.05).Conclusions: The expression of TGF-β1,uPA mRNA and proteins is high in GISTs,and TGF-β1 level positively correlates with that of uPA.They may play an important role in the genesis and development of GISTs.The results are contribution to further treatment.

关键词(KeyWords): 胃肠道间质瘤;反转录聚合酶链反应;免疫印迹法;转化生长因子β1;尿激酶型纤溶酶原激活物;TGF-β;uPA
gastrointestinal stromal tumors;RT-PCR;immunoblotting;TGF-β;uPA

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 赵滢;张天彪;王强;
ZHAO Ying1,ZHANG Tianbiao2,Wang Qiang1(1.Department of Gastro-intestinal Nutrition Surgery,the Affiliated Shengjing Hospital of China Medical University,Shenyang 110004,Liaoning,China;2.Department of Biochemistry and Molecular Biology,China Medical University,Shenyang 110001,Liaoning,China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2011.02.024

参考文献(References):

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享