申雪慧子,吴宁,陈朝军,陈红,谭宗建
SHEN Xuehuizi,WU Ning,CHEN Chaojun,CHEN Hong,TAN Zongjian

• 1:贵州省人民医院生殖中心
• 2:贵州医科大学基础医学院

摘要(Abstract)：

目的:探讨饮酒对不育男性精液质量的影响。方法:499例行精液常规及精子形态检查不育男性患者,按是否饮酒分为不育饮酒组(n=269)及不育非饮酒组(n=230),同期已生育的394例正常男性作为正常生育组(饮酒量<20 g/d),不育饮酒组按乙醇含量分为低(<20 g/d,n=91)、中(20～90 g/d,n=95)及高酒量组(> 90 g/d,n=83);所有受检者禁欲3～7 d,手淫方式收集精液,比较各组受检者精液量与液化时间;取各组受检者液化后精液0. 05 m L,采用beions3全自动精子质量分析系统检测精子总数、前向运动精子、精子浓度及精子活力;采用精子染色液对精子染色后,观察精子形态、计算精子正常形态率。结果:不育非饮酒组和不育饮酒组受检者的精子总数、精子浓度、向前运动精子、精子活力及正常形态率分别低于正常生育组(P <0. 05),不育饮酒组前向运动精子、精子活力、正常形态率低于不育非饮酒组(P <0. 05);高、中酒量组受检者的精子浓度、向前运动精子、精子活力及正常形态率低于不育非饮酒组(P <0. 05),低酒量组受检者的向前运动精子、精子活力及正常形态率低于不育非饮酒组(P <0. 05),中酒量组受检者的精子浓度、向前运动精子、精子活力及正常形态率高于高酒量组(P <0. 05),低酒量组向前运动精子、精子活力及正常形态率分别高于中、高酒量组(P <0. 05)。结论:饮酒导致男性精液质量降低,可能是导致男性生育力下降甚至不育的重要原因之一。
Objective: To investigate the effect of alcohol drinking on the semen quality of infertile Guiyang male. Methods: 499 infertile male patients underwent routine semen and sperm morphology check were divided into infertile drinking group( n = 269) and infertile non-drinking group( n =230). 394 health man( with children) were chosen as the normal fertility group( drinking amount <20 g/d). In terms of alcohol consumption,the infertile drinking group was further divided into low alcohol consumption group( < 20 g/d,n = 91),medium alcohol consumption group( 20-90 g/d,n= 95) and high drinking group( > 90 g/d,n = 83). All subjects abstained from sex for 3 to 7 days.Semen were collected by masturbation,and compared semen volume and liquefaction time between each group. 0. 05 m L liquefied semen from each group were analyzed: using beions3 automatic sperm quality analysis system to detect the total number of sperm, forward motility sperm, sperm concentration and sperm motility. At the same time,sperms were stained with sperm staining solution,and its morphology were observed and calculated the normal morphological rate of sperm. Results: The total number of sperm,sperm concentration,forward-moving sperm,sperm motility,and normal morphological rate of subjects in the infertile non-drinking group and infertile drinking group were lower than those of the normal fertility group( P < 0. 05). The forward-moving sperm,sperm motility,and normal morphology rate of sperm from sterile drinking group was lower than that of infertile nondrinking group( P < 0. 05); the sperm concentration,forward-moving sperm,sperm motility,and normal morphology rate of the high and medium alcohol groups were lower than that in the non-drinking group( P < 0. 05); the forward-moving sperm,sperm motility,and normal morphological rate of lowalcohol group were lower than that of infertile non-drinking group( P < 0. 05). The sperm concentration,forward-moving sperm,sperm motility and normal morphology rate of medium alcohol group was higher than that of high-alcoholic group( P < 0. 05). The forward-moving sperm,sperm motility and normal morphology rate in low-alcoholic group was higher than that of medium and high-alcoholic group( P <0. 05). Conclusion: Drinking alcohol leads to decreased quality in male semen,which may be one of the important reasons for the decline of male fertility,and even contributed to infertility.

关键词(KeyWords)： 不育;饮酒;精液分析;精子;贵阳市;男性
infertility;alcohol drinking;semen analysis;spermatozoa;guiyang city;male

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术厅科技计划项目[黔科合LH字(2014)7035]

作者(Author): 申雪慧子,吴宁,陈朝军,陈红,谭宗建
SHEN Xuehuizi,WU Ning,CHEN Chaojun,CHEN Hong,TAN Zongjian

DOI: 10.19367/j.cnki.2096-8388.2020.09.015

参考文献(References)：

• [1]LAMAESTRA S,DE F S,MICALE R T. Effect of drinking on DNA damage,oxidative stress,and spermatozoa[J].Hyg Environ Health,2015,2(18):117-122.
• [2]BONNICI J,FENECH A,MUSCAT C,et al. The role of seminal fluid in infertility[J]. Minerva Ginecol,2017,69(4):390-401.
• [3]曾强．饮用水消毒副产物暴露与男性精液质量关系的研究[D]．武汉:华中科技大学，2015.
• [4]陈卿．睡眠行为、昼夜节律与男(雄)性精液参数的关联研究[D]．重庆:第三军医大学，2016.
• [5]王琛．饮酒对男性不育患者精液质量及精子DNA完整性的影响分析[J]．中国优生与遗传杂志，2013,21(8):139-140.
• [6]刘丹，曹博玲，王海燕，等．饮酒对精液质量影响的Meta分析[J]．重庆医科大学学报，2013,38(12):1449-1452.
• [7]向小军，罗涛，李荣国，等．国内3个城市综合医院急诊饮酒模式和伤害风险的相关性研究[J]．中南大学学报，2016,41(9):992-997.
• [8]曾育鑫，钟达财．不育男性患者精液常规检测及影响精液质量下降的相关因素分析[J]．预防医学，2020,27(3):27-31.
• [9]王晓明，孙振高，杨毅，等．不良生活习惯对男性精液质量影响的研究进展[J]．中国性科学，2018,26(8):118-120.
• [10]冯阿洋．60例男性不育症患者的流行病学特点及相关影响因素[J]．中国卫生工程学，2020,18(15):64-66.
• [11]周平，宗晓涵，赵玉华，等．不良生活习惯对男性精液质量的影响[J]．中国医药科学，2015,25(22):330-333.
• [12]世界卫生组织．世界卫生组织人类精液检查与处理实验室手册[M]. 5版，北京:人民卫生出版社，2010.
• [13]颜秋霞，冼英杰，周秀琴，等．广东清远地区男性不育症患者精液质量与年龄的关系[J]．广东省医学会，2014,35(11):1390-1394.
• [14]江元元，徐望明．影响男性精液质量的因素分析[J]．中国生育健康杂志，2018,29(2):178-182.
• [15]洪菲，陈建伟，娄江涛，等．吸烟对精液质量和精子DNA完整性的影响[J]．中国现代医生，2015,53(26):12-18.
• [16]刘琳，李发敏子，张瑞，等．甘肃地区1705例不育门诊男性患者精液质量及影响因素分析[J]．中国男科学杂志，2019,19(6):17-22.
• [17]徐文杰，黄官友，周从容．待孕妇女年龄和获卵数对卵胞浆内单精子注射治疗结局的影响[J]．贵阳医学院学报，2015,8(11):1210-1213.
• [18]夏凤琼，黄健，杨国珍．三聚氰胺对雄性小鼠精液质量的影响[J]．贵阳医学院学报，2015,9(3):253-256.
• [19]赵良清，胡俊奇，谢凤坚，等．番禺区孕前检查男性精液质量及影响因素调查[J]．实用临床医学，2020,24(1):87-91.
• [20]莫金桦，赵瑰丽，李思慧，等．2005—2014年不孕不育门诊男性人群精液质量的单中心研究[J]．生殖医学杂志，2018,27(4):368-371.
• [21]张剑波，尹国良，徐新蓉，等．不育门诊就诊男性精液质量与影响因素分析[J]．生殖医学杂志，2015,24(12):1041-1044.
• [22]赵乾程，姜辉，唐文豪，等．影响供精志愿者精液质量的研究[J]．中国性科学，2018,34(8):42-45.
• [23]周侃，陈志刚，陈月婵．石河子地区6 178例男性精液质量评估及其与年龄的相关性探讨[J]．中国优生与遗传杂志，2018,26(4):104-106.
• [24]江元元，徐望明．影响男性精液质量的因素分析[J]．中国生育健康杂志，2018,29(2):178-182.
• [25]邱峰龙，倪蓉，仲纪祥，等．不育男性不同影响因素与精子质量参数的关系研究[J]．中国性科学，2017,26(5):112-115.
• [26]汪成，范舒舒，张思，等．2019年粤北地区男性精液质量分析[J]．中国现代药物应用，2020,14(8):30-32.
• [27]张剑波，尹国良，徐新蓉，等．不育门诊就诊男性精液质量与影响因素分析[J]．生殖医学杂志，2015,24(12):1041-1044.
• [28]阮衍泰，潘连军，赵丹，等．南京市孕前健康体检男性精液质量及其影响因素的初步调查分析[J]．中华男科学杂志，2015,21(2):144-148.

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