陆光辉;褚志华;曾守逵;李春美;杨洁;游浩;
LU Guanghui;ZHU Zhihua;ZENG Shoukui;LI Chunmei;YANG Jie;YOU Hao;Department of Laboratory, Minda Hospital Affiliated to Hubei University for Nationalities;The First Affiliated Hospital of Jianghan University;

• 1:湖北民族大学附属民大医院检验科
• 2:江汉大学第一附属医院

摘要(Abstract)：

目的:探讨类风湿性关节炎(RA)患者外周血Nod样受体蛋白3(NLRP3)炎症小体和白细胞介素1β(IL-1β)表达的变化及意义。方法:选取106例RA患者作为RA组、40例骨性关节炎患者作为非RA组、60例健康者作为对照组,RA与非RA组于入院后第2天清晨、对照组于体检当日清晨采集空腹静脉血检测C反应蛋白(CRP)、红细胞沉降率(ESR)、抗环瓜氨酸肽(CCP)抗体及类风湿因子(RF)水平;根据疾病活动性评分(DAS28)将RA患者分为高、中、低活动期,比较各个活动期RA患者外周血单个核细胞NLRP3、半胱氨酸蛋白酶1(Caspase-1)和凋亡相关斑点样蛋白(ASC)mRNA表达水平及血清IL-1β水平,采用Pearson分析RA患者外周血单个核细胞中NLRP3及血清IL-1β水平与其他临床指标相关性,采用多元线性回归分析DAS28评分影响因素。结果:与非RA组和对照组相比,RA组外周血单个核细胞中NLRP3、Caspase-1、ASC mRNA相对表达量及血清IL-1β水平均显著升高,且高度活动期>中活动期>低活动期(P<0.05);CRP、RF和NLRP3 mRNA表达量为RA患者DAS28评分的影响因素。结论:RA患者外周血单个核细胞中NLRP3 mRNA表达量和IL-1β与病情严重程度有关。
Objective: To investigate the expression and significance of nod-like receptor protein 3 inflammasome and interleukin 1β(IL-1β)in peripheral blood of patients with rheumatoid arthritis. Methods: 106 patients with RA were selected as RA group, 40 patients with osteoarthritis as non-RA group and 60 healthy subjects as control group.The fasting venous blood samples of RA and non-RA groups were collected on the morning of the second day after admission, and the fasting venous blood samples of the control group were collected on the morning of the day of physical examination. The samples were used to detect the C-reactive protein(CRP), ESR, anti-cyclic citrullinated peptide(CCP) antibody and rheumatoid factor(RF) level.Patients with RA were divided into high, middle and low activity stages according to disease activity score(DAS28).The expression of NLRP3,Caspase-1 and ASC mRNA in peripheral blood mononuclear cells and the level of serum IL-1β in patients with RA were compared.Pearson was used to analyze the correlation between NLRP3 and serum IL-1β levels in peripheral blood mononuclear cells of RA patients and other clinical indexes. Multiple linear regression analysis was used to analyze the influencing factors of DAS28 score. Results: Compared with non-RA group and control group, the relative expression levels of NLRP3, Caspase-1 and ASC mRNA in peripheral blood mononuclear cells and the level of IL-1β of patients with RA increased significantly, and high activity period>medium activity period>low activity period(P<0.05). The expression of CRP, RP and NLRP3 mRNA were influencing factors of DAS28 score in patients with RA(P<0.05). Conclusion: The expression of NLRP3 mRNA and IL-1β in peripheral blood mononuclear cells of patients with RA are related to the severity of the disease.

关键词(KeyWords)： 类风湿性关节炎;炎症小体;Nod样受体蛋白3;白细胞介素1β;疾病活动性
rheumatoid arthritis;inflammasome;Nod-like receptor protein 3;interleukin 1β;disease activity

Abstract:

Keywords:

基金项目(Foundation): 湖北省卫生厅科研基金项目(JX6C-25)

作者(Authors): 陆光辉;褚志华;曾守逵;李春美;杨洁;游浩;
LU Guanghui;ZHU Zhihua;ZENG Shoukui;LI Chunmei;YANG Jie;YOU Hao;Department of Laboratory, Minda Hospital Affiliated to Hubei University for Nationalities;The First Affiliated Hospital of Jianghan University;

参考文献(References)：

• [1] GEROSA M,SCHIOPPO T,MERONI P L.Challenges and treatment options for rheumatoid arthritis during pregnancy[J].Expert Opin Pharmacother,2016,17(11):1539-1547.
• [2] ALAM J,JANTAN I,BUKHARI S N A.Rheumatoid arthritis:recent advances on its etiology,role of cytokines and pharmacotherapy[J].Biomed Pharmacother,2017,92(8):615-633.
• [3] 何岱昆,邵义如,申捷,等.NLRP3炎症小体参与光气致急性肺损伤的炎症反应[J].中华劳动卫生职业病杂志,2017,35(7):491-496.
• [4] HOSEINI Z,SEPAHVAND F,RASHIDI B,et al.NLRP3 inflammasome:its regulation and involvement in atherosclerosis[J].J Cell Physiology,2018,233(3):2116-2132.
• [5] 潘美娟,徐胜前,王欣荣,等.血清成纤维生长因子23水平在类风湿关节炎及其骨质疏松中的临床研究[J].中华风湿病学杂志,2018,22(9):597-602.
• [6] 徐月辰,徐胜前,何秋时,等.体重指数和体脂百分比与类风湿关节炎的相关性研究[J].临床内科杂志,2018,35(1):46-49.
• [7] TEITSMA X M,JACOBS J W G,WELSING P M J,et al.Radiographic joint damage in early rheumatoid arthritis patients:comparing tocilizumab- and methotrexate-based treat-to-target strategies[J].Rheumatology,2018,57(2):309-317.
• [8] 张晓园,王永辉,李艳彦,等.JAK-STAT信号转导通路在类风湿性关节炎中的研究进展[J].山西中医学院学报,2016,17(2):63-65.
• [9] ZHU L,ZHU L.Sophocarpine suppress inflammatory response in human fibroblast-like synoviocytes and in mice with collagen-induced arthritis[J].Eur Cytokine Netw,2017,28(3):120-126.
• [10]MCALLISTER M J,CHEMALY M,EAKIN A J,et al.NLRP3 as a potentially novel biomarker for the management of osteoarthritis[J].Osteoarthritis Cartilage,2018,26(5):612-619.
• [11]何岱昆,邵义如,申捷,等.NLRP3炎症小体参与光气致急性肺损伤的炎症反应[J].中华劳动卫生职业病杂志,2017,35(7):491-496.
• [12]TANG T,GONG T,JIANG W,et al.GPCRs in NLRP3 inflammasome activation,regulation,and therapeutics[J].Trends Pharmacol Sci,2018,39(9):798-811.
• [13]GONG T,YANG Y,JIN T,et al.Orchestration of NLRP3 inflammasome activation by ion fluxes[J].Trends Immunol,2018,39(5):393-406.
• [14]MANGAN M S J,OLHAVA E J,ROUSH W R,et al.Targeting the NLRP3 inflammasome in inflammatory diseases[J].Nat Rev Drug Discov,2018,17(8):588-606.
• [15]CAI X H,YANG S P,SHEN H L,et al.Application of contrast-enhanced ultrasonography and ultrasonography scores in rheumatoid arthritis[J].Int J Clin Exp Med,2015,8(11):20056-20064.
• [16]王玉辉.类风湿性关节炎患者血清Th17/Treg细胞变化特征[J].北华大学学报(自然科学版),2018,19(1):53-55.

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