吴镭,毛久凤,郭艺,曾筱,李治,王碧超,董强
WU Lei,MAO Jiufeng,GUO Yi,ZENG Xiao,LI Zhi,WANG Bichao,DONG Qiang

• 1:贵州医科大学口腔医学院口腔修复学教研室
• 2:贵州医科大学附属口腔医院口腔内科
• 3:贵州医科大学
• 4:贵州医科大学附属省人民医院口腔修复科

摘要(Abstract)：

目的:研究成骨细胞膜片在两种不同改性钛表面上碱性磷酸酶(ALP)的表达。方法:原代培养SD大鼠成骨细胞,分别在阳极氧化处理钛表面(实验组)及光滑钛表面(对照组)上构建成骨细胞膜片,连续培养7d和14 d,采用ALP活性检测初步评价成骨细胞膜片在两种钛表面的成骨效应。结果:通过SD大鼠原代成骨细胞培养、纯化和鉴定,其形态学特征符合成骨细胞特性;将成骨细胞接种在两种钛表面,在第7天或第14天时实验组ALP活性均高于对照组(P<0.05),两组成骨细胞膜片第14天ALP活性均低于第7天(P<0.05)。结论:阳极氧化相对于光滑处理的钛表面而言,具有更显著的促进成骨细胞膜片成骨分化的作用。
Objective:To investigate the expression of alkaline phosphatase( ALP) of osteoblast cell sheets on two kinds of titanium surface. Methods:To culture primary osteoblasts of SD rats,then build osteoblast cell sheets 7 Days and 14 Days on the titanium surface of anodic oxidation( experimental group) and smooth( control group) respectively. By using ALP activity detection to preliminary evaluate the osteogenesis effect of osteoblast cell sheets on two kinds of titanium surface. Results:Through primary osteoblasts cultivation,purification and identifying,the morphology characteristics conformed to the osteoblasts. On the 7 Day or the 14 Day,the experimental group ALP activity was higher than that of the control group( P < 0. 05). Both groups of osteoblast cell sheets ALP activity on the 14~(th) Day were lower than that on the 7~(th) Day( P < 0. 05). Conclusion:The anodic oxidation titanium surface has a more significant promoting effect on the osteogenic promotion comparing with the smooth titanium surface.

关键词(KeyWords)： 成骨细胞;细胞培养;碱性磷酸酶;阳极氧化钛表面;光滑钛表面
osteoblast cell sheets;cell culture;alkaline phophatase;anodic oxidation titanium surface;smooth titanium surface

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅省校合作协议项目[黔科合LH字(2014)7115号];; 贵阳市科技计划项目[筑科合同(2014)100139号]

作者(Author): 吴镭,毛久凤,郭艺,曾筱,李治,王碧超,董强
WU Lei,MAO Jiufeng,GUO Yi,ZENG Xiao,LI Zhi,WANG Bichao,DONG Qiang

DOI: 10.19367/j.cnki.1000-2707.2017.03.018

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