王寒琪,王晋星一,陈林,陈妮,陈我婵,潘娅,洪阳,陈腾祥
WANG Hanqi1,WANG Jinxingyi2,CHEN Ning2,CHEN Ni1,CHEN Wochan2, PAN Ya2,HONG Yang3,CHEN Tengxiang2(1.Department of Gastroenterology

• 1:贵阳医学院附院消化内科
• 2:贵州省电力职工医院
• 3:贵阳医学院生理学教研室
• 4:贵阳市卫生局

摘要(Abstract)：

目的:观察Nanog、Sox2和Oct4干细胞相关基因在四株不同迁移能力肝癌细胞系中的表达。方法:常规细胞培养无迁移能力的HepG2细胞、低迁移能力的MHCC97-L细胞、中迁移能力的LM3细胞及高迁移能力的MHCC97-H细胞,采用免疫荧光组织化学方法观察四株肝癌细胞系中肝癌干细胞相关基因Nanog、Sox2和Oct4的表达。结果:干细胞相关基因Nanog、Oct4和Sox2在四株迁移能力不同的肝癌细胞系中不同程度表达,且在细胞膜、细胞核、细胞浆中的表达量亦不同;Nanog在细胞核表达,并随着细胞迁移能力增强而呈递增趋势;Sox2在不同迁移能力肿瘤细胞中表达无明显规律,而Oct4在高迁移能力肝癌细胞的细胞浆及核中高表达。结论:Nanog、Sox2和Oct4在不同迁移能力肝癌细胞系中均有表达,但无明显规律,推测肝癌干细胞与肝癌的发生可能有密切关系。
Objective: To observe the expression of stem cell related genes Nanog,Sox2 and Oct4 in 4 hepatoma cell lines with different migration abilities.Methods: HepG2(no migration ability),MHCC97-L(lowly migratory),LM3(moderately migratory),and MHCC97-H(highly migratory) respectively were routinely cultured.Immunofluorescent cytochemical technique was employed to observe the expression of liver cancer stem cell related genes Nanog,Sox2 and Oct4 in 4 liver cancer cell lines.Results: Nanog,Sox2 and Oct4 expressed in 4 cell lines in different degrees,and the expression levels in the cell membrane,nucleus,cytoplasm were different.The expression of Nanog showed an increasing trend in the nucleus with the increase of cell migration abilities.Sox2 expression had no obvious rule in the 4 cell lines.Oct4 highly expressed in cytoplasm and nucleus of cells with high migration abilities.Conclusions: The expressions of Nanog,Sox2 and Oct4 have no obvious rule in the 4 hepatoma cell lines with different migration abilities.However,Nanog,Sox2 and Oct4 are all detectable in the 4 cell lines,suggesting that liver cancer stem cells is closely related to the occurrence of liver cancer.

关键词(KeyWords)： 干细胞基因;肝癌细胞;肝癌干细胞;Nanog;Sox2;Oct
Stem cell gene;hepatoma cells;liver cancer stem cells;Nanog;Sox2;Oct4

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅自然科学基金项目[2008]2280号;; 贵州省教育厅自然科学研究项目(2008)022号;; 贵阳市科技局社发攻关项目(2010)1号

作者(Author): 王寒琪,王晋星一,陈林,陈妮,陈我婵,潘娅,洪阳,陈腾祥
WANG Hanqi1,WANG Jinxingyi2,CHEN Ning2,CHEN Ni1,CHEN Wochan2, PAN Ya2,HONG Yang3,CHEN Tengxiang2(1.Department of Gastroenterology

DOI: 10.19367/j.cnki.1000-2707.2013.03.003

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