夏琍;耿晓照;朱延韦;蒋宪瑤;张爱华;
Xia Li Institute of Traditional Chinese Medicine, Guizhou

• 1: 贵州省中医研究所药理室
• 2:贵州省中医研究所药理室
• 3:贵阳医学院卫生系
• 4:贵阳医学院卫生系

摘要(Abstract)：

对贵州省抗矽肺新药——异汉防己甲素进行了诱变性、致癌性系列筛检实验。包括Ames试验、微核试验、姐妹染色单体互换(SCE)试验、精于畸形试验和显性致死试验,结果均为阴性。说明本品无致突变作用,致癌的可能性亦较小。
A series of sereening assays on the mutagenicity and carcinogenicity were performed. In Ames plate-incorporation assay, no mutagenic effects were found at the dose up to 500 μg/plate, in which the strains TA_(97) TA_(98) TA_(100) and TA_(102) were used. Isotetrandrine 225, 450, 900mg/kg per os did not induce any increment of micronucleus rate in mouse bone mallow cells. The frequencies of sister chromatid exchanges in mouse bone mallow cells in vivo were weakly positive at 120, 150mg/kg, and in a dosage of 18 or 54 μg/ml in the human peripheral blood lymphocytes cultured in vitro, it was found that the frequncies of SCE was increase significantly. For sperm malformation assay, administered 112.5, 225, 450 mg/kg for 5 days in male mice, the increased rates of abnormal sperm formation (5.6, 6.4, 5.2‰) were insignificant as compared to controls (3.0‰). The result of dominant lethal study showed that no increases of early fetal deaths and mutation index were noticed in female mice, which had matched with males, when adminis- tered 18, 60, 180mg/kg for 5 days.

关键词(KeyWords)： 异汉防己甲素;生物碱类;诱变性试验;致癌物;基因;姐妹染色单体互换;精子
isotetrandrine; alkaloids; mutagenicity tests; eareinogens; genes; sister chromatid exchange; spermatozoa

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 夏琍;耿晓照;朱延韦;蒋宪瑤;张爱华;
Xia Li Institute of Traditional Chinese Medicine, Guizhou

DOI: 10.19367/j.cnki.1000-2707.1989.02.005

参考文献(References)：

• 1． 汉防己甲素治疗矽肺研究协作组。汉防己甲素治疗矽肺作用原理的研究及汉防己甲素治疗早期单纯性矽肺的临床疗效观察。中华劳动卫生职业病杂志 1983;3(1) :133～136。
• 2． 朱延韦,等。异汉防己甲素毒性研究。中华劳动卫生职业病杂志1985;3(1) :34。
• 3． Bruce N Ames, et al. Revised methods for the Sslmoella mutagenicity. Mutation Res 1983; 113: 173．
• 4． 贺维顺,等。一种简易的姐妹染色单体分化试验--紫外线照射加吉姆薩染色(UPG)。自然杂志1980;3(3) :638。
• 5． Myrobek AJ, et al. 关于非人类哺乳动物精子试验的评价。 国外医学卫生学分册 1984;(5) :269。
• 6． Donald A, et al. Statistical models for mumber of implantation sites and embryonic deaths in mice. Toxicol Appl Pharmacol 1976; 38(2) : 325．

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