赵文静,漆一鸣
ZHAO Wenjing,QI Yiming(Department of Biology

• 1:贵阳医学院生物学教研室

摘要(Abstract)：

目的:从分子水平探讨方形黄鼠蚤七河亚种(Citellophilus tesquorum dzetysuensis)和阿尔泰亚种(C.t.altaicus)的分类地位。方法:用苯酚-氯仿法抽提单蚤胸段DNA,PCR扩增获得二亚种核糖体RNA基因(rD-NA)的两段内转录间隔序列(Internal Transcribed Spacer)ITS1和ITS2,分析比对序列差异。结果:阿尔泰亚种ITS1序列长1458bp,第508、783和1197位存在碱基差异;七河亚种ITS1序列长1452bp,亚种内无差异;两亚种间ITS1序列存在碱基差异,分别位于第508位(颠换A→C)和第783位(转换C→T),七河亚种从第1344到1349位缺失6bp,两亚种ITS2序列均长317bp,亚种内、亚种间均无差异。结论:七河亚种可能独立于阿尔泰亚种。
Objective:To study classification positions of Citellophilus tesquorum altaicus Ioff,1936 and Citellophilus tesquorum dzetysuensis Mikulin,1951 at molecular level. Methods:Genomic DNA from the thorax of single flea was isolated with Phenol-Chloroform method. Sequences of ITS1 and ITS2 in rDNA of the two subspecies were amplified with PCR. An analysis was made on the results. Results:The ITS1 sequence of C. t. altaicus was 1 458 bp,in which three base differences happened at the positions 508,783 and 1 197 at intra-subspecific level. The ITS1 sequence of C. t. dzetysuensis was 1 452 bp,in which no difference presented at intra-subspecific level. Two base substitutions at inter-subspecific level appeared at positions 508 (transversion,A→C) and 783 (transition,C→T). C. t.dzetysuensis lacked 6 bp from the position 1 344 to 1 349. ITS2 sequences in both subspecies were 317 bp with no difference at neither intra-subspecific nor inter-subspecific level. Conclusion:The results may support that C.t. dzetysuensis is a subspecies independent from subspecies C. t. altaicus.

关键词(KeyWords)： 蚤;序列分析,DNA;碱基序列
fleas; sequence analysis,DNA; base sequence

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 赵文静,漆一鸣
ZHAO Wenjing,QI Yiming(Department of Biology

DOI: 10.19367/j.cnki.1000-2707.2010.03.009

参考文献(References)：

• [1]Vobis M,D'Haese J,Mehlhorn H,et al.Molecular phy-logeny of isolates of Ctenocephalides felis and related spe-cies based on analysis of ITS1,ITS2and mitochondrial16S rDNA sequences and random binding primers[J].Parasitol Res,2004(3):219-226.
• [2]Vobis M,D'Haese J,Mehlhorn H,et al.Molecular bio-logical investigations of Brazilian Tunga sp.isolates fromman,dogs,cats,pigs and rats[J].Parasitol Res,2005(2):107-112.
• [3]崔耀仁,郑楠,任凤岐,等.黄鼠蚤实体标本与玻片标本体长测量结果[J].中国地方病防治杂志,2009(2):139.
• [4]Gamerschlag S,Mehlhorn H,Heukelbach J,et al.Re-petitive sequences in the ITS1region of the ribosomal DNA of Tunga penetrans and other flea species(Insecta,Siphonaptera)[J].Parasitol Res,2008(2):193-199.
• [5]鲁亮,吴厚永.新蚤属9种新蚤核糖体DNA-ITS2序列变化[J].寄生虫与医学昆虫学报,2002(2):106-113
• [6]Luchetti A,Mantovani B,Pampiglione S,et al.Molecu-lar characterization of Tunga trimamillata and T.pene-trans(Insecta,Siphonaptera,Tungidae):taxonomy and genetic variability[J].Parasite,2005(2):123-129.
• [7]Luchetti A,Trentini M,Pampiglione S,et al.Genetic variability of Tunga penetrans(Siphonaptera,Tungidae)sand fleas across South America and Africa[J].Parasitol Res,2007(3):593-598.
• [8]吴厚永.中国动物志.昆虫纲.蚤目[M].2版.北京:科学出版社,2007:2170-2174.

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