吴昌学,任家谋,官志忠,齐晓岚
WU Changxue,REN Jiamou,GUAN Zhizhong,QI Xiaolan

• 1:贵阳医学院分子生物学重点实验室
• 2:贵阳市第四人民医院检验科
• 3:贵阳医学院病理学教研室

摘要(Abstract)：

目的:研究β淀粉样肽(Aβ)处理SH-SY5Y细胞以及α3神经型尼古丁受体(α3 nAChR)沉默细胞后细胞Notch信号通路的变化。方法:2μmol/L Aβ处理SH-SY5Y细胞以及α3 nAChR沉默细胞,用实时荧光定量聚合酶链式反应(Real Time PCR)和蛋白印迹(western blot)方法分别检测细胞中Notch信号通路中Notch1和Hes1的mRNA及蛋白水平的变化,评价Aβ对细胞Notch信号通路的影响。结果:Aβ处理的SH-SY5Y细胞以及α3 nAChR沉默细胞中Notch1和Hes1的mRNA及蛋白水平表达均增加,且在α3 nAChR沉默细胞中增加更为明显,差异有统计学意义(P<0.05)。结论:Aβ可增加细胞Notch信号通路基因表达,α3 nAChR沉默会增强Aβ对Notch信号通路基因表达的影响,这可能与阿尔茨海默氏病(AD)的发病有一定的关系。
Objective: To observe the changes of Notch signal transduction pathway in SH-SY5Y neuroblastoma cells and 3 nAChR silence cells after treated with β-amyloid peptide( Aβ). Methods: SH-SY5Y cells and 3 nAChR silence cells were exposed to 2 μmol / L Aβ1- 42. The mRNA and protein levels of Hes1 and Notch1 of Notch signal transduction pathway were measured by using Real-Time PCR and Western Blotting methods respectively. Results: mRNA and protein levels of Notch1 and Hes1 increased both in Aβ treated SH-SY5Y cells and 3 nAChR silence cells,and specially in 3 nAChR silence cells,the increase was more obvious. The differences were statistically significant( P < 0. 05). Conclusions: Aβ may enhance the gene expression in Notch signal transduction pathway; and the silence in α3 nAChR cells can enhance such effects,which might be connected to the pathogenesis of AD.

关键词(KeyWords)： β淀粉样肽;Notch信号通路;α3神经型尼古丁受体;阿尔茨海默氏病;Notch1;Hes1;基因表达
β-amyloid peptide;Notch signal pathway;3 nAChR;alzheimer's disease;Notch1;Hes1;gene expression

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81360178);; 贵州省科技厅社会发展攻关项目(黔科合SY字[2013]3020号);; 优秀青年人才项目(黔科合人字(2013)44号);; 贵州省教育厅优秀科技人才项目(黔教合KY字[2012]089号)

作者(Author): 吴昌学,任家谋,官志忠,齐晓岚
WU Changxue,REN Jiamou,GUAN Zhizhong,QI Xiaolan

DOI: 10.19367/j.cnki.1000-2707.2014.02.004

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