赵燕;杨文秀;冯江龙;
ZHAO Yan;YANG Wenxiu;FENG Jianglong;Department of Pathology, Guizhou Medical University;Department of Ophthalmology, Guizhou People's Hospital;

• 1:贵州医科大学临床医学院病理学教研室
• 2:贵州省人民医院眼科
• 3:贵州医科大学

摘要(Abstract)：

目的:探讨多发性骨髓瘤基因(MUM1)蛋白与恶性黑素瘤(MM)的可溶性蛋白S100、Melan-A蛋白、Y染色体性别决定区相关高迁移率盒10蛋白(SOX10)及黑色素瘤单克隆抗体45(HMB45)蛋白表达之间的关系。方法:MM病例手术切除组织标本60例,采用免疫组化法检测MUM1、S100、Melan-A、SOX10及HMB45蛋白的表达,并分析MUM1与S100、Melan-A、SOX10及HMB45蛋白的相关性。结果:60例MM组织标本中,MUM1、S100、Melan-A、HMB45和SOX10蛋白阳性率分别为51.7%、86.66%、85.00%、93.33%和80.00%;MM组织标本中MUM1蛋白阳性表达与Melan-A蛋白的阳性表达相关有高度统计学意义(P<0.01),但与S100、SOX10、HMB45蛋白的阳性表达相关无统计学意义(P>0.05)。结论:MUM1蛋白可作为MM鉴别诊断的细胞免疫表型之一,对MM的诊断和鉴别诊断具有一定的价值。
Objective: To detect the expression of multiple myeloma oncogene 1(MUM1), S100, melan-A, SOX10 and HMB45 in malignant melanoma(MM), so as to discover the correlation between the expression of MUM1 and other frequently-used labelled proteins in antidiastole of MM. Methods: Immunohistochemistry was used to detect the expression of MUM1, S100, melan-A, SOX10 and HMB45 proteins in 60 cases with MM. Results: The expression of MUM1 protein had significant difference from that of melan-A(P<0.05), but no significant difference was found in the expression of S100, SOX10 and HMB45(P>0.05). Conclusion: MUM1 protein can be an immunophenotypic in antidiastole of MM. Meanwhile, it has potential value in the diagnosis and antidiastole of MM.

关键词(KeyWords)： 多发性骨髓瘤;基因,肿瘤;黑色素瘤;免疫组织化学;蛋白;免疫表型分型
multiple myeloma;genes,neoplasm;malignant melanoma;immunohistochemistry;protein;immunophenotyping

Abstract:

Keywords:

基金项目(Foundation): 贵阳市科技局贵州医科大学联合基金(GY2016-4)

作者(Authors): 赵燕;杨文秀;冯江龙;
ZHAO Yan;YANG Wenxiu;FENG Jianglong;Department of Pathology, Guizhou Medical University;Department of Ophthalmology, Guizhou People's Hospital;

DOI: 10.19367/j.cnki.1000-2707.2019.10.016

参考文献(References)：

• [1] PAUL K,LEISLI E,SOBI N,et al.廖松林译.WHO皮肤肿瘤病理学和遗传学[M].3版.北京:人民卫生出版社,2006.
• [2] 郭胜,芦佳娜.眼部恶性黑色素瘤40例临床病理分析[J].中国基层医药,2012,19(24):3693-3694.
• [3] CSCO黑色素瘤专家委员会.中国黑色素瘤诊治指南(2011版)[J].临床肿瘤学杂志,2012,17(2):159-171.
• [4] 苏月颖,安秀梅,赵华,等.调节性T细胞和CD8～+T细胞在恶性黑色素瘤中的表达及其与预后的关系[J].天津医药,2015,23(9):1026-1030.
• [5] KRIEGSMANN M,KRIEGSMANN K,HARMS A,et al.Expression of HMB45,MelanA and SOX10 is rare in non-small cell lung cancer[J].Diagnostic Pathology,2018,13(1):68-69.
• [6] SAXTON G D,NEELY D G.The relationship between sarbanes-oxley policies and donor Advisories in nonprofit organizations[J].Journal of Business Ethics,2018,(2):1-19.
• [7] WEISSINGER S E,LENNERZ J K.Comparison of melana/mart-1 and hmb45 labeling in desmoplastic melanoma[J].Mod Pathol,2014,27(10):1421-1423.
• [8] LIU H G,KONG M X,YAO Q,et al.Expression of Sox10 and c-kit in sinonasal mucosal melanomas arising in the Chinese population[J].Head Neck Pathol,2012,6(4):401-408.
• [9] POSCH C,MOSLEHI H,SANLOREZO M,et al.Pharmacological inhibitors of c-kit block mutant c-kit mediated migration of melanocytes and melanoma cells in vitro and in vivo[J].Oncotarget,2016,7(29):45916-45925.
• [10]CRONIN J C,WATINS-CHOW D E,INCAO A,et al.SOX10 ablation arrests cell cycle,induces senescence and suppresses Melan omagenesis[J].Cancer Res,2013,73(18):5709-5718.
• [11]车江雁,王宝宏.BCL2、BCL6、MUM1蛋白表达及其与弥漫性大B细胞淋巴瘤预后的关系[J].现代医药卫生,2016,32(1):25-27.
• [12]TSUBOI K,IIDA S,INAGAKI H,et al.MUM1/IRF4 expression as a frequent event in mature lymphoid malignancies[J].Leukemia,2000,14(3):449- 456.
• [13]FALINI B,FIZZOTTI M,PUCCIARINI A,et al.A monoclonal antibody(MUM1p) detects expression of the MUM1/IRF4 protein in a subset of germinal center B cells,plasma cells,and activated T cells[J].Blood,2000,95(6):2084- 2092.
• [14]徐傲,陈柯,王琦,等.恶性黑色素瘤11例临床细胞病理学分析[J].安徽卫生职业技术学院学报,2017,16(4):107-109.
• [15]曹军,邱建学,李春飞,等.阴道无色素梭形细胞型恶性黑色素瘤1例临床病理分析[J].临床与实验病理学杂志,2016,32(2):217-218.
• [16]THOMAS J M,NEWTON BISHOP J,AHERN R,et al.Excision margins in high-risk malignant melanoma[J].The New England Journal of Medicine,2004,8(8):757-766.
• [17]WEISSINGER S E,LENNERZ J K.Comparison of Melana/Mart-1 and HMB45 labeling in desmoplastic melanoma [J].Mod Pathol,2014,27(10):1421-1423.
• [18]SATZGER I,VOLKER B,MEIER A,et al.Prognostic significance of isolated HMB45 or Melan a positive cells in melanoma sentinel lymph nodes [J].Am J Surg Pathol,2007,31(8):1175-1180.
• [19]孙妍,张清媛,张明辉,等.外周T细胞淋巴瘤MUM1/IRF4蛋白的表达及其临床意义[J].临床肿瘤学杂志,2012,17(3):215-218.
• [20]DZWIERZYNSKI W W.Managing malignant melanoma[J].Plast Reconstr Surg,2013,132(3):446-460.
• [21]李腾政,罗文,张吉翔.SOX10在黑色素瘤中的作用[J].实用医学杂志,2015,1(19):3267-3268.
• [22]CAYREFOURCQ L,DE ROECK A,GARCIA C,et al.S100-EPISPOT:a new tool to 22 detect viable circulating melanoma cells[J].Cells,2019,8(7):E755.
• [23]RONCATI L,PISCIOLI F,PUSIO T,et al.Microinvasive radial growth phase of cutaneous melanoma:a histopathological and immunohistochemical study with diagnostic implications[J].Acta Dermatovenerol Croat,2017,25(1):39-45.
• [24]KRIEGSMANN M,KRIEGSMANN K,HARMS A.Expression of HMB45,MelanA and SOX10 is rare in non-small cell lung cancer[J].Diagn Pathol,2018,13(1):68.
• [25]DANGA M E,YAAR R,BHAWAN J.Melan-A positive dermal cells in malignant melanoma in situ[J].J Cutan Pathol,2015,42(6):388-393.
• [26]YIN H,QIN C,ZHAO Y,et al.SOX10 is over-expressed in bladder cancer and contributes to the malignant bladder cancer cell behaviors[J].Clinical & Translational Oncology,2017,19(8):1035-1044.
• [27]ORDó?EZ N G.Value of melanocytic-associated immunohistochemical markers in the diagnosis of malignant melanoma:a review and update[J].Hum Pathol,2014,45(2):191-205.

文章评论(Comment)：