张丽欣,孙丽芳,刘毅,邢利和,张丽丽,李娜,石雪华
ZHANG Lixin,SUN Lifang,LIU Yi,XING Lihe,ZHANG Lili,LI Na,SHI Xuehua

• 1:中国人民解放军第二六一医院

摘要(Abstract)：

目的:不同时间给予尼莫地平治疗血管性痴呆(VD)小鼠,观察小鼠脑组织氧自由基水平的变化。方法:将健康昆明小鼠随机分为假手术组、模型组、尼莫地平造模后即刻给药组、尼莫地平造模3 d后给药组,后3组小鼠反复结扎双侧颈动脉同时加尾部放血制作VD模型,两个尼莫地平给药组分别于造模后即刻、造模后3 d灌服尼莫地平,模型组及假手术组均每日3次灌服注射用生理盐水,治疗10 d后检测4组小鼠脑组织丙二醛(MDA)含量及SOD活性的变化。结果:模型组小鼠脑组织中MDA含量比假手术组显著增高(P<0.05),尼莫地平两个治疗组小鼠脑组织中MDA含量均明显低于模型组(P<0.05),且尼莫地平造模后即刻给药组MDA含量比尼莫地平造模3 d后给药组更低(P<0.05);模型组小鼠中SOD活性比假手术组显著降低;尼莫地平两个治疗组脑组织中SOD活性较模型组显著增高,且尼莫地平造模后即刻给药组比尼莫地平造模3 d后给药组更高(P<0.05)。结论:尼莫地平能降低痴呆小鼠体内MDA含量,提高SOD活性,即刻给药组的疗效优于3 d后给药组,所以VD一旦确诊应尽快给予尼莫地平干预,以预防病情的进一步发展。
Objective:To observe the changes of oxygen free radical metabolism in brain tissue of vascular dementia mice caused by nimodipine administered at different time points. Methods:Forty healthy Kunming mice were randomly divided into 4 groups:sham-operation group( group S),model group( group M),and nimodipine treated groups( group N1,received nimodipine on day 0,and group N2 received nimodipine on day 3). The models of vascular dementia mice were induced by repeated ligation of bilateral common carotid arteries and tail bloodletting. The mice in groups N1 and N2 were respectively given nimodipine on 0,and 3rd days after the models were established,and mice in groups S and M were given normal saline instead. Ten days after treatment,the contents of oxygen free radical metabolism in mice brain tissue were measured. Result:The content of oxygen free radical malondialdehyde( MDA) was significantly higher in group M than in group S( P < 0. 05),lower in two nimodipine treatment groups than in group M( P < 0. 05),and significantly lower in group N1 than in group N2( P < 0. 05). The activity of oxygen free radical superoxide dismutase( SOD) was significantly lower in group M than in group S( P < 0. 05),higher in two nimodipine treatment groups than in group M( P < 0. 05) and higher in group N1 than in group N2( P < 0. 05). Conclusion:Nimodipine can decrease the content of MDA in mice with dementia,and improve the activity of SOD,and the curative effect of the immediate treatment group was better than that of the 3-days later treatment group. Therefore,once vascular dementia is diagnosed patient should be treated immediately,so as to obtain better therapeutic effect.

关键词(KeyWords)： 痴呆,血管性;尼莫地平;氧自由基;丙二醛;小鼠
dementia vascular;nimodipine;oxygen free radical;malondialdehyde;mice

Abstract:

Keywords:

基金项目(Foundation): 河北省中医药管理局科研计划项目(2014199)

作者(Author): 张丽欣,孙丽芳,刘毅,邢利和,张丽丽,李娜,石雪华
ZHANG Lixin,SUN Lifang,LIU Yi,XING Lihe,ZHANG Lili,LI Na,SHI Xuehua

DOI: 10.19367/j.cnki.1000-2707.2017.03.013

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