韩晓曦,武迪迪,宗志红,于秉治
HAN Xiaoxi,WU Didi,ZONG Zhihong,YU Bingzhi(Department of Biochemistry and Molecular Biology

• 1:中国医科大学基础医学院生物化学与分子生物学教研室
• 2:中国医科大学基础医学院生物化学与分子生物学教研室
• 3:中国医科大学基础医学院生物化学与分子生物学教研室
• 4:中国医科大学基础医学院生物化学与分子生物学教研室 辽宁沈阳110001

摘要(Abstract)：

目的:研究磷酸二酯酶3A(PDE3A)在小鼠受精卵第1次有丝分裂中的表达。方法:采用逆转录多聚酶链式反应(RT-PCR)方法检测小鼠受精卵在第1次有丝分裂的G1、S、G2及M期,在mRNA水平PDE3A的表达。结果:在mRNA水平,PDE3A在G2、M期的表达较G1、S期明显增高。结论:小鼠受精卵中有PDE3A基因的表达;在小鼠受精卵第1次有丝分裂过程中,PDE3A在mRNA水平的表达具有阶段特异性,因而,PDE3A很可能参与对受精卵早期发育的调控。
Objective: To research the expression of phosphodiesterase 3A(PDE3A) in zygote of mouse during the first mitosis.Methods: The expression of PDE3A in mRNA level during the periods of G1,S,G2,and M of the cell cycle was examined by RT-PCR.Results: The expression of PDE3A in phases of G2 and M was significantly stronger than that in phases of G1 and S.Conclusions:(1) PDE3A expresses in one-cell stage of mouse fertilized eggs;(2) During the first mitosis of mouse zygote,the expression pattern of PDE3A is different during early stages of embryo development and shows phase specificity,which suggests the PDE3A activity could contribute to the regulation of early development of mouse fertilized egg to some extent.

关键词(KeyWords)： 磷酸二酯酶类;有丝分裂;逆转录聚合酶链反应;小鼠;受精卵
phosphodiesterases;mitosis;reverse transcriptase polymerase chain reaction;mice;zygote

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(基金编号:30570945)

作者(Author): 韩晓曦,武迪迪,宗志红,于秉治
HAN Xiaoxi,WU Didi,ZONG Zhihong,YU Bingzhi(Department of Biochemistry and Molecular Biology

DOI: 10.19367/j.cnki.1000-2707.2007.03.005

参考文献(References)：

• [1]Bingzhi Yu,Yajie Wang,Ying Liu,et al.Protein kinaseA regulates cell cycle progression of mouse fertilized eggsby means of MPF[J].Developmental Dynamics,2005(232):98-105.
• [2]Nekola MV,Smith DM.Failure of gonadotropins to inducein vitro maturation of mouse oocyte treated with dibutytylcyclic AMP[J].J Exp Zool,1975(194):529-533.
• [3]Urner F,Herymann WL,Baulieu EE,et al.Inhibition ofdenuded mouse oocyte meiotic maturation by forskolin,anactivator of adenylate cyclase[J].Endocrinology,1983(113):1170-1172.
• [4]Eppig JJ.The participation of cyclic adenosine monophos-phate(cAMP)in the regulation of meiotic maturation of o-ocytes in the laboratory mouse[J].J Reprod Fertil Suppl,1989(38):3-8.
• [5]Finidori-Lepicard J,Schorderet-Slatkine S,Hanoune J,etal.Progesterone inhibits membrane-bound adenylate cycla-se in Xenopus laevis oocytes[J].Nature,1981(292):255-257.
• [6]Maller JL,Butcher FR,Krebs EG.Early effect of proges-terone on levels of cyclic adenosine 3’5’-monophosphatein Xenopus oocytes[J].J Biol Chem,1979(254):579-582.
• [7]Huchon D,Ozon R,Fischer EH,et al.The pure inhibitorof cAMP-dependent protein kinase initiates Xenopus laevismeiotic maturation.A4-step scheme for meiotic maturation[J].Mol Cell Endocrinol,1981(22):211-222.
• [8]Brian C Duckworth,Jennifer S Weaver,Joan V.Ruder-man.G2 arrest in Xenopus oocytes depends on phosphoryl-ation of cdc25 by protein kinase A[J].Proc Natl AcadSci,2002(99):16794-16799.
• [9]Stambrook PJ,Velez C.Reversible arrest of Chinese ham-ster V79 cells in G2 by dibutytyl AMP[J].Exp Cell Res,1976(99):57-62.
• [10]Kurokawa K,Kato J.Cyclic AMP delays G2 progressionand prevents efficient accumulation of cyclin B1 proteinsin mouse macrophage cells[J].Cell Struct Funct,1998(23):357-365.
• [11]Conti M,Jin S L.The molecular biology of cyclic nucleo-tide phosphodiesterases[J].Prog Nucleic Acid Res MolBiol,1999(63):1-38.
• [12]Thomas RE,Armstrong DT,Gilchrist RB.Differentialeffects of specific phosphodiesterase isoenzyme inhibitorson bovine oocyte meiotic maturation[J].Dev Biol,2002(44):215-225.
• [13]Bilodeau-Goeseels S.Effects of phosphodiesterase inhibi-tors on spontaneous nuclear maturation and cAMP con-centrations in bovine oocytes[J].Theriogenology,2003(60):1679-1690.
• [14]Sadler SE.Inhibitors of phosphodiesteraseⅢblock stim-ulation of Xenopus laevis oocyte ribosomal S6 kinase ac-tivity by insulin-like growth factor-I[J].Mol Endocrinol,1991(5):1947-1954.
• [15]Shitsukawa K,Andersen CB,Richard FJ,et al.Cloningand characterization of the cGMP-inhibited phosphodies-terase PDE3A expressed in mouse oocyte[J].Biol Re-prod,2001(65):188-196.
• [16]Masciarelli S,Horner K,Liu C,et al.Cyclic nucleotidephosphodiesterase 3A-deficient mice as a model of femaleinfertility[J].J Clin Invest,2004(114):196-205.
• [17]郑洁,武迪迪,王雁,等.小鼠受精卵早期发育过程中M期促进因子的活性研究[J].中国医科大学学报,2000(29):3-5.

文章评论(Comment)：