贵州医科大学学报

2014, v.39;No.169(04) 494-497

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

阈下电刺激对大鼠缺血心肌细胞凋亡及bcl-2、bax表达的影响
Effects of Subthreshold Electrical Stimulation on Cardiomyocyte Apoptosis and Gene Expression of bcl-2 and bax in Ischemic Cardiomyocytes of Rats

杨峰;沈祥春;何艳;张蓓;沈正;刘兴德;
YANG Feng;SHEN Xiangchun;HE Yan;ZHANG Bei;SHEN Zheng;LIU Xingde;Department of Cardiology,the Affiliated Hospital of Guiyang Medical College;Department of Pharmacology,Guiyang Medical College;Ultrasound Center,the Affiliated Hospital of Guiyang Medical College;

摘要(Abstract):

目的:探讨阈下电刺激对大鼠缺血心肌细胞凋亡及bcl-2和bax表达的影响。方法:Wistar大鼠随机分为假手术组、急性心梗组、阈下电刺激组,假手术组手术不结扎,置入电极不给予电刺激;急性心梗组和阈下电刺激组采用左冠状动脉前降支结扎法建立大鼠急性心肌梗死模型,急性心梗组仅植入电极,不给予电刺激,阈下电刺激组在建立心肌梗死模型后,给予25 Hz、0.3 V阈下电刺激,TUNEL法检测3组大鼠心肌细胞凋亡,免疫组化和RT-PCR的方法检测心肌bcl-2及bax蛋白和mRNA的表达。结果:(1)与假手术组比较,急性心梗组、阈下电刺激组大鼠心肌细胞凋亡指数显著升高(P<0.05);与急性心梗组比较,阈下电刺激组大鼠心肌细胞凋亡指数降低(P<0.05)。(2)与假手术组比较,急性心梗组、阈下电刺激组大鼠心肌细胞bcl-2和bax蛋白和mRNA的表达显著升高(P<0.05);与急性心梗组比较,阈下电刺激组大鼠心肌细胞bcl-2蛋白和mRNA的表达显著升高,而bax基因表达显著降低(P<0.05)。结论:阈下电刺激能显著减少大鼠心肌梗死后缺血心肌细胞凋亡,其机制可能与上调bcl-2表达和下调bax表达有关。
Objective: To explore the effects of subthreshold electrical stimulation on cardiomyocytes apoptosis and gene expression of bcl-2 and bax in ischemic cardiomyocytes of rats. Methods: 18 rats were randomly divided into 3 groups:sham operation group( group S),acute myocardial infarction(AMI) group(group A) and subthreshold electrical stimulation group(group E). The left anterior descending coronary of rat hearts in group A and group E were ligated to establish AMI model. Electrodes were implanted into rats in the three group,but not giving electrical stimulation in group S and group A,and rats in group E were given subthreshold electrical stimulation of 25 Hz and 0. 3. Cardiomyocyte apoptosis was detected with TUNEL method,and the expressions of bcl-2 and bax were determined by using immunohistochemistry and RT-PCR methods. Results:(1) Compared with group S,the apoptotic indexes of cardiomyocytes in group A and group E were increased significantly( P <0. 05);Compared group A,the apoptotic indexes of cardiomyocytes in group E were decreased significantly(P < 0. 05).(2) Compared with group S,the expressions of bcl-2 and bax gene and protein in group A and group E were increased significantly(P < 0. 05); Compared with that of group A,the expression of bcl-2 gene and protein in group E was increased significantly,while the expression of bax gene and protein was decreased significantly(P < 0. 05). Conclusions: Subthreshold electrical stimulation can reduce the number of apoptotic cardiomyocytes in AMI rats,the mechanisms may be associated with up-regulating the expression of bcl-2 and down-regulating the expression of bax.

关键词(KeyWords): 电刺激;心肌梗塞;细胞凋亡;基因,bcl-2;大鼠,Wistar
electrical stimulation;myocardial infarction;cell apoptosis;genes,bcl-2;rats,Wistar

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金项目黔科合丁字[2007]2089号

作者(Author): 杨峰;沈祥春;何艳;张蓓;沈正;刘兴德;
YANG Feng;SHEN Xiangchun;HE Yan;ZHANG Bei;SHEN Zheng;LIU Xingde;Department of Cardiology,the Affiliated Hospital of Guiyang Medical College;Department of Pharmacology,Guiyang Medical College;Ultrasound Center,the Affiliated Hospital of Guiyang Medical College;

Email:

DOI: 10.19367/j.cnki.1000-2707.2014.04.013

参考文献(References):

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享