梁文妹,李一欣,夏白娟,潘贵书
LIANG Wenmei1,LI Yixin1,XIA Baijuan1,PAN Guishu2(1.Department of Histology and Embryology

• 1:贵阳医学院组织学与胚胎学教研室
• 2:遵义医学院生理学教研室

摘要(Abstract)：

目的:探讨海洛因依赖对大鼠垂体远侧部β-内啡肽(β-endrophin,β-EP)表达的影响。方法:成年雄性SD大鼠55只,随机分为正常对照组、盐水对照组及海洛因依赖组;皮下注射海洛因建立海洛因依赖大鼠模型,分别于模型建立的第10、17、24、31、38天取垂体组织,应用免疫组织化学SABC法、图像分析法观察大鼠垂体远侧部β-内啡肽表达。结果:与正常对照组和盐水对照组比较,海洛因依赖组大鼠垂体远侧部β-EP免疫反应明显减弱,平均灰度值增高(P<0.05)。结论:在海洛因依赖期间,垂体远侧部β-EP表达减少,提示海洛因依赖使内源性阿片样物质的产生受到抑制。
Objective:To investigate the effect of heroin dependence on the expression of β-endrophin(β-EP)in pars distalis hypophyseos for exploring the possible mechanism of the changes.Methods:Fifty-five male adult SD rats were randomly divided into a normal control group(group NCG),a saline control group(group SCG)and a heroin dependence group(group HDG).Heroin-dependent rat models were set up by subcutaneous injection of heroin and the hypophysis were excised on the 10th,17th,24th,31th,and 38th days after models were set up.Immunohistochemical SABC method,imagine analysis,and radioimmunoassay were used in the research.Results:Compared with those of groups NCG,and SCG,the immune staining intensity of β-EP cells in group HGD was weaker,and the average gray value was significantly higher(P<0.05).Conclusions:β-EP positive cells diminish during heroin dependence,The morphological and functional changes suggest that heroin inhibit the production of endogenous opioid.

关键词(KeyWords)： 海洛因依赖;β内啡肽;垂体;免疫组织化学;大鼠,Sprague-Dawley
heroin dependence;beta-endorphin;pituitary gland;immunohistochemistry;rats,Sprague-dawley

Abstract:

Keywords:

基金项目(Foundation): 贵州省优秀科技人才省长基金(2005)14号;; 高层次人才基金(2007)26号

作者(Author): 梁文妹,李一欣,夏白娟,潘贵书
LIANG Wenmei1,LI Yixin1,XIA Baijuan1,PAN Guishu2(1.Department of Histology and Embryology

DOI: 10.19367/j.cnki.1000-2707.2009.06.009

参考文献(References)：

• [1]Soverchia L,Mosconi G,Ruggeri B,et al.Proopiomel-anocortin gene expression and beta-endorphin localizationin the pituitary,testis,and epididymis of stallion[J].Mol Reprod Dev,2006(1):1-8.
• [2]Shi J,Li SX,Zhang XL,et al.Time-dependent neuroen-docrine alterations and drug craving during the first month of abstinence in heroin addicts[J].Am J Drug Alcohol Abuse,2009(27):1.
• [3]杨国栋,顾钧,叶明珠,等.东莨菪碱对吗啡成瘾大鼠下丘脑、垂体及血β-内啡肽和催产素含量的影响[J].中华医学杂志,1995(1):8-10.
• [4]叶晓明,王新华,李家乐,等.海洛因依赖和戒断时大鼠脑区、垂体和血浆中β-内啡肽的变化[J].中华麻醉学杂志,1999(12):733-735.
• [5]潘贵书,徐国强,李淑芳,等.海洛因成瘾大鼠模型的建立[J].贵阳医学院学报,1997(增刊):46-46.
• [6]韩济生.神经科学纲要[M].北京:北京医科大学中国协和医科大学联合出版社,1993:465.
• [7]Fukuda Y,Kageyama K,Nigawara T,et al.Effects of corticotropin-releasing hormone(CRH)on the synthesis and secretion of proopiomelanocortin-related peptides in the anterior pituitary:a study using CRH-deficient mice[J].Neurosci Lett,2004(2):201-204.
• [8]李红,杨良,徐仕文,等.海洛因依赖者下丘脑-垂体-肾上腺轴功能改变[J].中国内分泌代谢杂志,1995(2):86-87.
• [9]杜雨苍.神经肽与脑功能[M].上海:上海科技教育出版社,1998:160-161.
• [10]杨良,李红.海洛因依赖基础医学与临床医学[M].昆明:云南科技出版社,1998:33.
• [11]Bronstein DM,Przewlocki R,Akil H.Effects of mor-phine treatment on pro-opiomelanocortin systems in rat brain[J].Brain Res,1990(1-2):102-111.

文章评论(Comment)：