严志凌,杨宏英,张红平,尹春梅,张芍,李文亮,李传印,史荔,杨谢兰
YAN Zhiling,YANG Hongying,ZHANG Hongping,YIN Chunmei,ZHANG Shao,LI Wenliang,Li Chanyin,SHI Li,YANG Xielan

• 1:昆明医科大学第三附属医院
• 2:中国医学科学院&北京协和医学院医学生物学研究所

摘要(Abstract)：

目的:探讨CD25基因多态性与云南汉族人群宫颈癌发生发展的相关性。方法:选取云南地区汉族人群癌前病变(CIN)患者136例,宫颈癌患者227例以及汉族健康体检人群483例,采用Taq Man探针基因分型方法对CD25基因中SNP位点rs7072793(C>T)和rs3118470(C>T)进行基因分型,研究其等位基因、基因型及所构建的单倍型在CIN患者、宫颈癌患者和健康对照人群中分布频率。结果:rs7072793(C>T)等位基因C和T在CIN组和对照组、CIN组与癌症组中分布频率的差异具有统计学意义(P=0.039和0.039),提示该位点等位基因C在宫颈癌发生和发展阶段分别发挥保护(OR=0.75;95%CI∶0.57~0.99)和风险因素(OR=1.38;95%CI∶1.02~1.86);rs3118470(G>T)的等位基因C和T在CIN组和对照组、CIN组和癌症组中分布频率的差异具有统计学意义(P<0.001和P<0.001),提示该位点等位基因C在宫颈癌发生和发展阶段分别发挥保护(OR=0.576;95%CI∶0.436~0.762)和风险因素(OR=1.750;95%CI∶1.283~2.388)。结论:CD25基因中多态性位点rs7072793(C>T)和rs3118470(C>T)的等位基因C是宫颈癌发生的保护性因素和宫颈癌发展过程中的风险因素。
Objective: To investigate the correlation of CD25 gene polymorphism with the occurrence and development of cervical cancer in a Yunnan Han population. Methods: A total of 136 patients with CIN,227 patients with cervical cancer and 483 healthy individuals were recruited in this study.Taq Man probe genotyping was adopted to analyze the genotypes of SNP locus rs7072793( C > T) and rs3118470( C > T) of CD25 gene and to research the frequencies of the alleles,genotypes and haplotypes in CIN patients,cervical cancer patients and healthy controls. Results: CIN group < cancer group < control group in terms of C allele frequency distribution of rs7072793( C > T) and CIN group >cancer group > control group in terms of T allele frequency distribution of rs7072793( C > T),the differences were statistically significant( P < 0. 05). The allele C of rs7072793( C > T) was a protective factor in stage of initiation of cervical cancer( OR = 0. 75,95% CI∶ 0. 57 ~ 0. 99) and risk factor in developmental stage of cervical cancer( OR = 1. 38,95% CI∶ 1. 02 ~ 1. 86). CIN group < cancer group or control group in terms of C allele frequency distribution of polymorphic site rs3118470( C >T) and CIN group > cancer group or control group in terms of T allele frequency distribution of polymorphic site rs3118470( C > T). There were statistically significant differences between CIN group and cancer group or control group( P < 0. 001). The allele C of rs3118470( C > T) was a protective factor in stage of initiation of cervical cancer( OR = 0. 576,95% CI∶ 0. 436 ~ 0. 762) and risk factor in developmental stage of cervical cancer( OR = 1. 750,95% CI∶ 1. 283 ~ 2. 388). Conclusion: In polymorphic sites of rs7072793( C > T) and rs3118470( C > T) of CD25 gene,C allele is protective factor of cervical cancer and risk factor in the development of cervical cancer.

关键词(KeyWords)： 基因型;宫颈肿瘤;云南汉族;CD25;多态性;相关性
genotype;cervical neoplasm;Yunnan Han population;CD25;polymorphisms;correlation

Abstract:

Keywords:

基金项目(Foundation): 云南省医学学科带头人培养计划(D-201669);; 云南省科技厅-昆明医科大学应用基础研究联合专项(2013FB064);云南省科技厅-昆明医科大学应用基础研究联合专项(201501UH01630);; 云南省肿瘤内设机构(2014NS139);; 国家自然科学基金项目(81573206);; 云南省妇科肿瘤内设机构(2014NS032,2014NS033)

作者(Author): 严志凌,杨宏英,张红平,尹春梅,张芍,李文亮,李传印,史荔,杨谢兰
YAN Zhiling,YANG Hongying,ZHANG Hongping,YIN Chunmei,ZHANG Shao,LI Wenliang,Li Chanyin,SHI Li,YANG Xielan

DOI: 10.19367/j.cnki.1000-2707.2017.04.001

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