谭艳,宁丽常,蹇孝丽,蒋红梅
TAN Yan,NING Lichang,JIAN Xiaoli,JIANG Hongmei

• 1:贵州医科大学医学检验学院临床微生物免疫教研室
• 2:长沙医学院医学检验学院临床微生物免疫教研室
• 3:贵州中医学院第一附属医院检验科

摘要(Abstract)：

目的:探讨胶原诱导性关节炎(CIA)大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6)、干扰素-γ(IFN-γ)和IL-10水平。方法:取Sprague Dawley(SD)雌性大鼠16只,随机均分为CIA组和对照组,CIA组大鼠以牛Ⅱ型胶原(BⅡC)行左足跖部注射,对照组同法注射生理盐水;造模后第7、14、21、28、35及42天观察大鼠足趾关节炎症症状并评定关节炎指数,第42天处死大鼠后取左后足踝关节进行苏木精-伊红(HE)染色组织学观察,同时取血采用酶联免疫吸附试验(ELISA)检测血清TNF-α、IL-6、IFN-γ及IL-10表达水平。结果:与对照组相比,CIA组大鼠踝关节以下肿胀非常明显并伴有畸形,关节炎指数升高但随时间延长呈先高后低趋势(P<0.05),HE染色见关节处炎症病理病变明显;造模第42天时,CIA组大鼠血清IL-10水平较对照组降低,TNF-α、 IL-6及IFN-γ水平较对照组升高(P<0.05)。结论:CIA大鼠血清TNF-α、IL-6及IFN-γ水平的升高,IL-10水平的降低可能与RA的发生发展密切相关。
Objective: To investigate levels of tumor necrosis factor α(TNF-α), interleukin 6(IL-6), interferon γ(IFN-γ) and IL-10 in the serum of collagen-induced arthritis(CIA) rats. Methods: 16 SD female rats were randomly divided into two groups(8 in each group) as CIA and control group. The rats in the CIA group received injection of bovine type Ⅱ collagen(BⅡC) on the sole of their left paw, control group received saline injection; observing rats' toe joint arthritis on 7, 14, 21, 28, 35 and 42 day after modeling, then evaluating the index of arthritis. On the 42~(nd) day, the rats were sacrificed. HE staining was performed on the posterior ankle joint of their left foot. Enzyme-linked immunosorbent assay( ELISA) was used to detect the expression levels of TNF-α, IL-6, IL-10 and IFN-γ. Result: Compared with the control group, swelling of the ankle joints in the CIA group was obvious and accompanied by malformation, the arthritis index increased(P<0.05), and HE staining showed pathological lesions of the joints. Serum IL-10 levels were decreased in the CIA group, and TNF-α, IL-6 and IFN-γlevels were increased(P<0.05). Conclusion: The increased levels of serum TNF-α, IL-6 and IFN-γ, together with decreased IL-10 level are closely correlated to the occurrence and development of RA.

关键词(KeyWords)： 肿瘤坏死因子-α;白细胞介素-6;干扰素-γ;白细胞介素-10;胶原诱导性关节炎;牛Ⅱ型胶原
tumor necrosis factor-α;interleukin-6;Interferon-gamma;interleukin-10;collagen-induced arthritis;bovine type Ⅱ collagen

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金地区科学基金(81660361,81960393)

作者(Author): 谭艳,宁丽常,蹇孝丽,蒋红梅
TAN Yan,NING Lichang,JIAN Xiaoli,JIANG Hongmei

DOI: 10.19367/j.cnki.1000-2707.2020.01.004

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