蔡辉,张群燕,姚茹冰
CAI Hui,ZHANG Qunyan,YAO Rubing

• 1:南京军区南京总医院

摘要(Abstract)：

目的:探讨类风湿关节炎(RA)患者外周血单核细胞(PBMCs)Toll样受体(TLR)2、4mRNA和蛋白的表达及脂多糖(LPS)对其表达的影响。方法:收集已确诊的RA患者5例(DAS28≥2.6),健康自愿者5例;体外分别分离培养受试者PBMCs作为RA组和健康组,下设空白组与LPS组,其中LPS组给予100μg/L LPS进行刺激,细胞培养24 h后,FQ-RT PCR检测单核细胞TLR2、TLR4 mRNA表达;Western blot检测单核细胞TLR2、TLR4蛋白表达。结果 :给予LPS刺激后,RA组与健康组PBMCs TLR2、TLR4 mRNA及其蛋白的表达均高于相应组刺激前(P<0.001);给予LPS刺激前和刺激后RA组PBMCs TLR2、TLR4 mRNA及其蛋白的表达水平均明显高于健康组(P<0.001)。结论:LPS可刺激RA患者PBMCs已高表达TLR2、TLR4的表达水平更高,可能与机体的炎症反应有关。
Objective: To explore the effects of lipopolysaccharide( LPS) on Toll-like receptor(TLR) 2,4 mRNA and protein expression in peripheral blood mononuclear cells( PBMCs). Method: Five RA patients( DAS28 ≥2. 6) and 5 healthy volunteers were recruited,PBMCs were isolated and cultured in vitro as RA group and healthy group,under the two group,there were control group and LPS group,LPS group was given 100 μg/L LPS for stimulation,the cells were cultured for 24 h,FQ-RT PCR was employed to detect TLR2,TLR4 mRNA expression; Western blot was used to detect of TLR2,TLR4 protein expression. Results: Compared with the healthy group,TLR2,TLR4 mRNA and protein expression levels in PBMCs of RA group were significantly increased( P < 0. 001); after administration of LPS,compared with the control group,TLR2,TLR4 mRNA and protein expression in PBMCs of LPS group were increased,and the change of RA group was more significant( P <0. 001). Conclusion: LPS can stimulate higher expression of TLR2,TLR4 expression levels in PBMCs of RA patients higher whose TLR2,TLR4 expressions are already at high level,which may relate to inflammatory response.

关键词(KeyWords)： 脂多糖;类风湿关节炎;Toll样受体;单核细胞;基因表达;细胞培养
lipoplysaccharide;rheumatoid arthritis;Toll-like receptor;mononuclear cells;gene expression;cell culture

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年科学基金项目(81303124)

作者(Author): 蔡辉,张群燕,姚茹冰
CAI Hui,ZHANG Qunyan,YAO Rubing

DOI: 10.19367/j.cnki.1000-2707.2017.01.015

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