高秀丽,王鹏娇,彭安堂,刘祉莉,刘安发
GAO Xiuli1,WANG Pengjiao1,PENG Antang1,LIU Zhili2,LIU Anfa1(1.School of Pharmacology

• 1:贵阳医学院药学院
• 2:贵州省肿瘤医院

摘要(Abstract)：

目的:建立紫珠果药材中齐墩果酸和熊果酸的含量测定方法。方法:采用RP-HPLC法,Phenomenex Kromasil ODS-Cl8(4.6 mm×250 mm,5μm)色谱柱;流动相为甲醇-水(90∶10);流速1.0 ml/min;柱温25℃;检测波长为215 nm。结果:齐墩果酸在0.011 5~0.103 5 g/L呈良好的线性关系;熊果酸在0.030 32~0.272 88g/L呈良好的线性关系;齐墩果酸平均回收率为99.70%,RSD为0.32%(n=9),熊果酸平均回收率为100.06%,RSD为0.87%(n=9)。结论:该方法操作简便快速,结果可靠,重复性好,可为有效控制紫珠果药材及其制剂的质量提供依据。
Objective: To establish a high performance liquid chromatography(HPLC) method for determination of oleanolic acid and ursolic acid in Zizhu fruit.Methods: The HPLC method was carried on C18(4.6 mm×250 mm,5 μm)column using methanol-water(90∶10,V:V) as the mobile phase,and detection wavelength was 215 nm.The flow rate was 1.0 ml/min and the temperature of column 25 ℃.Results: The calibration curve for oleanolic acid was linear in the range of 0.011 5～0.103 5 g/L,and the calibration curve for ursolic acid was linear in the range of 0.030 32～0.272 88 g/L,The average recovery of oleanolic acid was 99.70%,and RSD was 0.32%(n=9),while average recovery of ursolic acid was 100.06%,RSD 0.87%(n=9).Conclusions: The method is convenient,precise,reliable and with good reproducibility for determining the contents of oleanolic acid and ursolic acid in Zizhu,which may provide basis for effective quality control of Zizhu fruit products.

关键词(KeyWords)： 色谱法,高压液相;齐墩果酸;熊果酸;紫珠果
chromatography,high pressure liquid;oleanolic acid;ursolic acid;Zizhu fruit

Abstract:

Keywords:

基金项目(Foundation): 贵阳市科技局基金项目(合同号:[2007]筑科计合同字第6-15号)

作者(Author): 高秀丽,王鹏娇,彭安堂,刘祉莉,刘安发
GAO Xiuli1,WANG Pengjiao1,PENG Antang1,LIU Zhili2,LIU Anfa1(1.School of Pharmacology

DOI: 10.19367/j.cnki.1000-2707.2010.04.008

参考文献(References)：

• [1]杨新河,刘仲华,黄建安,等.HPLC法测定苦丁茶中的熊果酸和齐墩果酸的含量[J].药物分析杂志,2007(10):1627.
• [2]张晓喻,黄春萍,张宏,等.RP-HPLC测定四川不同产地枇杷花中齐墩果酸和熊果酸的含量[J].药物分析杂志,2007(1):129.
• [3]刘伟,丁海杰.HPLC测定夏枯草中熊果酸、齐墩果酸、迷迭香酸的含量[J].中成药,2008(4):577.
• [4]王建,罗诚,齐红艺,等.HPLC测定复方大红袍止血片中熊果酸的含量[J].华西药学杂志,2006(2):200.
• [5]李江.反相高效液相色谱法测定橘红中柚皮苷和柚皮含量[J].实用临床医药杂志,2009(19):30-32.
• [6]解加扬,张国欣,景崤璧.高效液相色谱法测定氯尼达明胶囊的含量[J].实用临床医药杂志,2007(09):41-43.

文章评论(Comment)：