王霞,肖海涛,齐晓岚,郝小燕
WANG Xia1,XIAO Haitao1,QI Xiaolan2,HAO Xiaoyan1 (1.School of Pharmacology

• 1:贵阳医学院药学院
• 2:贵阳医学院分子生物学重点实验室

摘要(Abstract)：

目的:探讨微乳薄层色谱法在虎杖及其成方制剂分离鉴定中的应用。方法:以聚酰胺薄膜为固定相,SDS-正丁醇-正庚烷-水微乳液作为展开剂,采用大黄素、大黄素甲醚、大黄酚、大黄素苷、白藜芦醇及白藜芦醇苷为对照品,对虎杖及其成方制剂进行微乳薄层色谱研究。结果:以含水量为75%的微乳液-甲酸(9∶1)为展开剂,能够使虎杖及其成方制剂的定性鉴别取得满意的效果,与普通薄层色谱比较,微乳薄层色谱展出的斑点数多,分离度好。结论:本研究结果为虎杖及其成方制剂的定性鉴别提供了一种简便、准确、可靠的方法。
Objective:To investigate the application value of microemulsion thin-layer chromatograph(TLC) in identification of Rhizoma Polygoni Cuspidati and its prescription preparation.Methods:Microemulsion TLC was carried out by using polyamide thin film as stationary phase,SDS-n-butanol-n-heptane-water microemulsion as developer,and emodin,physcione,chrysophanol,emodin-8-O-β-D-glucopyranosid,resveratrol and polydatin as control articles.Results:The best developer for identifying Rhizoma Polygoni Cuspidati and its prescription preparation was microemulsion-methanol(9∶1) of which the moisture content was 75%.Conclusions:Compared with the ordinary TLC,identification with microemulsion TLC shows more spots and better resolution.These results provide a simple,accurate and reliable way to study the quality of Rhizoma Polygoni Cuspidati and its prescription preparation.

关键词(KeyWords)： 色谱法,薄层;虎杖;中成药;中药鉴定
chromatography,thin layer;polygonum cuspidatum;ready-made patent drugs

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金项目,黔科合J字(2009)2143号

作者(Author): 王霞,肖海涛,齐晓岚,郝小燕
WANG Xia1,XIAO Haitao1,QI Xiaolan2,HAO Xiaoyan1 (1.School of Pharmacology

DOI: 10.19367/j.cnki.1000-2707.2011.03.006

参考文献(References)：

• [1]李干佐,郭荣.微乳液理论及其应用[M].北京:石油工业出版社,1995:59.
• [2]崔淑芳,林焕冰,Frank S C,等.微乳薄层色谱法鉴别甘草的研究[J].中草药,2007(4):540-543.
• [3]韩明,曾庆孝,肖更生,等.青梅黄酮类化合物的微乳薄层色谱分离鉴定[J].林产化学与工业,2006(6):53-56.
• [4]曾长青,谭泉基.止咳枇杷颗粒的微乳薄层鉴别[J].广东药学院学报,2006(3):261-262.
• [5]孙悦,张茜,毕静文,等.微乳薄层色谱用于糖的分离研究[J].化学分析计量,2010(1):75-77.
• [6]孙晓华,周玲芝.中药虎杖的研究进展[J].中医药导报,2009(5):107-110.
• [7]邬浩杰.两种中药黄酮类成分的微乳液薄层色谱鉴别[J].浙江中医药杂志,2010(1):52-54.
• [8]徐军,李凤.抗病毒颗粒的薄层色谱鉴别[J].中医中药,2008(3):245-246.
• [9]徐健,刘建中.对热炎宁颗粒质量标准修订的建议[J].华西药学杂志,2007(1):110.
• [10]唐华蕊,温怀清,张志刚.清消冲剂薄层鉴别定性研究[J].中华医药学杂志,2003(11):116-117.

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