贵州医科大学学报

2012, v.37;No.155(02) 152-155

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β-胡萝卜素对人膀胱癌T24细胞生物学行为的影响
Effects of β-carotene on Biological Behavior of Human Bladder Cancer Cell Line T24

吴志平;赵晓昆;吕晨;
WU Zhiping1,ZHAO Xiaokun2,LV Chen2(1.Department of Urology,the Affiliated Hospital Guiyang Medical College,Guiyang 550004, Guizhou,China;2.Department of Urology,the Second Xiangya Hospital of Central South University,Changsha 410011,Hunan,China)

摘要(Abstract):

目的:探讨β-胡萝卜素对人膀胱癌T24细胞细胞增殖和凋亡的影响。方法:不同浓度的β-胡萝卜素作用于T24细胞,MTT法检测细胞增殖,流式细胞术检测T24细胞的凋亡指数。结果:MTT法检测结果显示10μmol/L和20μmol/L组β-胡萝卜素均能明显抑制T24细胞的生长,抑制率分别为28.83%和63.02%(P<0.05);流式细胞术检测显示β-胡萝卜素能诱导T24细胞调亡,5μmol/L组T24细胞的凋亡指数为(0.065±0.018)(P<0.05),10μmol/L和20μmol/L组凋亡指数分别为(0.126±0.022)和(0.190±0.024)(P<0.01),呈现剂量依赖效应,10μmol/L组及20μmol/L组Hochest染色可见凋亡小体形成。结论:β-胡萝卜素抑制T24细胞的生长,并且呈现剂量依赖效应,其机制可能为诱导细胞凋亡。
Objective: To explore the effects of β-carotene on proliferation and apoptosis of human bladder cancer cell line T24.Methods: T24 cells were treated with different concentrations of β-carotene for 72 hours.Proliferation of T24 cells was tested with Hochest staining and MTT assay.T24 cell apoptosis was determined with flow cytometry.Results: MTT assay showed that β-carotene at concentrations of 10 μmol/L and 20 μmol/L could significantly inhibit T24 cell proliferation,and the inhibition rates were 28.83% and 63.02% respectively(P<0.05).Flow cytometry showed that β-carotene could induce T24 cell apoptosis,apoptotic index(AI) of 5 μmol/L group was 0.065±0.018(P<0.05),with the increase of β-carotene concentration,AI increased,AI of 10 μmol/L and 20 μmol/L groups were 0.126±0.022 and 0.190±0.024 respectively(P≤0.01),showing a dose-dependent manner.Apoptotic bodies were detectable when the cells stained by Hochest method.Conclusions: β-carotene could inhibit T24 cell proliferation in a dose-dependent manner,the mechanism may be associated with inducing T24 cell apoptosis.

关键词(KeyWords): β-胡萝卜素;癌;膀胱;肿瘤细胞,培养的;细胞凋亡;流式细胞术
beta-carotene;carcinoma;bladder;tumor cells,culture;apoptosis;flow cytometry

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作者(Author): 吴志平;赵晓昆;吕晨;
WU Zhiping1,ZHAO Xiaokun2,LV Chen2(1.Department of Urology,the Affiliated Hospital Guiyang Medical College,Guiyang 550004, Guizhou,China;2.Department of Urology,the Second Xiangya Hospital of Central South University,Changsha 410011,Hunan,China)

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DOI: 10.19367/j.cnki.1000-2707.2012.02.013

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