贵州医科大学学报

2006, (03) 221-222

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贵州省部分少数民族线粒体DNA 9bp序列缺失频率研究
An Analysis on the Frequency of 9bp Deletion of Mitochondrial DNA in the Ethnic Groups of Guizhou

齐晓岚;谢渊;单可人;吴昌学;赵艳;马骄;李毅;任锡麟;
QI Xiao-lan,XIE Yuan,SHAN Ke-ren~(**),WU Chang-xue,ZHAO Yan,MA Jiao,LI Yi,REN Xi-lin(Molecular Biological Key Laboratory of Guiyang Medical College,Guiyang 550004, China)

摘要(Abstract):

目的:研究中国贵州省部分少数民族线粒体DNA 9 bp序列缺失情况。方法:采用PCR法扩增线粒体基因组细胞色素氧化酶II和tRNAlys基因间非编码序列中含9bp序列缺失的片段,3%琼脂糖凝胶电泳检测缺失情况。结果:各少数民族线粒体DNA 9 bp序列缺失频率为7.4%~23.4%,最低为银屏壮族,最高为沙井苗族。结论:贵州省部分少数民族线粒体DNA 9bp序列缺失频率较高,各民族DNA 9 bp序列缺失频率有一定差异。
Objective: To analyze the frequency of 9bp deletion of mtDNA in the ethnic groups of Guizhou.Methods: Polymerase chain reaction was adopted to amplify the mtDNA un-coding segment between the cytochrome oxidase II and tRNALys containing the 9bp deletion.3% agarose gel electrophoresis was used to detect the deletion in the products.Results: The frequencies of 9 bp deletion in mtDNA of the ethnic groups in Guizhou were from 7.4% to 23.4%.Conclusions: The frequencies of 9 bp deletion in mtDNA in all the ethnic groups in Guizhou are relatively high,and there are some differences among the groups.

关键词(KeyWords): DNA,线粒体;序列缺失;少数民族,贵州
DNA,mitochondrial;sequence deletion;minority groups,Guizhou

Abstract:

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基金项目(Foundation): 2001年度贵州省省长专项基金(2001033);; 2002年度贵州省卫生厅基金资助项目

作者(Author): 齐晓岚;谢渊;单可人;吴昌学;赵艳;马骄;李毅;任锡麟;
QI Xiao-lan,XIE Yuan,SHAN Ke-ren~(**),WU Chang-xue,ZHAO Yan,MA Jiao,LI Yi,REN Xi-lin(Molecular Biological Key Laboratory of Guiyang Medical College,Guiyang 550004, China)

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DOI: 10.19367/j.cnki.1000-2707.2006.03.007

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