江二喜
JIANG Erxi

• 1:恩施土家族苗族自治州中心医院

摘要(Abstract)：

目的:探讨左归丸对免疫性卵巢早衰小鼠血清性激素及IL-6、IL-17水平的影响。方法:SPF级雌性小鼠60只随机分为对照组、模型组、西药组及左归丸组,除对照组外,所有雌鼠足底、腹部多点皮下注射免疫试剂0.15 m L,14 d后再次皮下注射免疫强化试剂0.15 m L,建立免疫性卵巢早衰模型;造模成功第6天,西药组灌胃补佳乐0.15 mg/kg,左归丸组灌胃31.5 g/kg左归丸,对照组和模型组灌胃等量的蒸馏水;各组雌鼠灌胃8周时,取清晨空腹静脉血,ELISA法检测血清中雌激素、卵泡刺激素、黄体生成素,及抗苗勒管激素、抗透明带抗体、基础抑制素B,及IL-6、IL-17水平;摘取雌鼠卵巢及子宫秤取湿重,计算小鼠卵巢系数、子宫系数。结果:与对照组比较,模型组卵巢系数、子宫系数及血清雌激素、抗苗勒管激素、基础抑制素B水平明显降低(P<0.01),卵泡刺激素、黄体生成素及抗透明带抗体、IL-6及IL-17水平明显升高(P<0.01);与模型组比较,西药组及左归丸组卵巢系数、子宫系数及血清雌激素、抗苗勒管激素、基础抑制素B水平明显升高(P<0.01),卵泡刺激素、黄体生成素及抗透明带抗体、IL-6及IL-17水平明显降低(P<0.01);左归丸组上述指标优于西药组(P<0.01)。结论:左归丸有效下调免疫性卵巢早衰小鼠IL-6、IL-17水平,增加生殖器官重量、改善激素水平,从而保护卵巢功能。
Objective: To investigate the effect of Zuogui pills on the serum sex hormones and IL-6 and IL-17 of the immune ovarian progeria mice. Methods: Sixty SPF female mice were randomly divided into control group,model group,western medicine group and Zuogui pill group. Except for the control group,all female mice were made subcutaneous injection of the foot and abdomen with 0. 15 mL of the immune reagent and then were made subcutaneous injection with 0. 15 mL of immune enhanced reagent again after 14 d to set up autoimmune ovarian premature senility model; 6 d after it,the western medicine group were filled stomach and lavage jiale 0. 15 mg/kg,zuogui pill group took 31. 5 g/kg of zuogui pill while control group and model group taking the same amount of distilled water. After all groups took eight weeks' continuous lavage,the morning fasting venous blood was taken to test the levels of estrogen,follicle stimulating hormone,luteinizing hormone,and anti-muller tube hormone,antihyaluronic band antibody,basal inhibitory hormone B,and IL-6,IL-17 in serum by ELISA. The ovary and uterus scales of female mice were extracted and the wet weight was taken to calculate the ovary coefficient and uterine coefficient. Results: Compared with control group,model group were significantly lower in levels of ovarian coefficient,uterine coefficient and serum estrogen,anti-muller tube hormone,basic inhibitory factor B( P < 0. 01),the levels of follicular stimulating hormone,luteinizing hormone and anti-transparent band antibody,IL-6 and IL-17 were significantly increased( P < 0. 01);compared with model group,western medicine group and Zuogui pill group were significantly increased in levels of ovarian coefficient,uterine coefficient and serum estrogen,anti-muller tube hormone,basic inhibitory factor B( P < 0. 01). The levels of follicular stimulating hormone,luteinizing hormone and anti-transparent band antibody,IL-6 and IL-17 significantly decreased,the above indexes of zuogui pill group were better than those of western medicine group( P < 0. 01). VConclusions: The Zuogui pill effectively reduces the level of IL-6 and IL-17 in the immune ovarian premature aging mice,increases the weight of the reproductive organs,promotes follicular development,improves the hormone level and protects the ovarian function.

关键词(KeyWords)： 左归丸;免疫性疾病;卵巢早衰;性激素;白细胞介素6;白细胞介素17
Zuogui pill;immune diseases;premature ovarian failure;sex hormones;interleukin 6;interleukin 17

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 江二喜
JIANG Erxi

DOI: 10.19367/j.cnki.1000-2707.2018.08.009

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