李铮,史荔,严志凌,李传印,刘舒媛,姚宇峰,刘峰
LI Zheng,SHI Li,YAN Zhiling,LI Chuanyin,LIU Shuyuan,YAO Yufeng,LIU Feng

• 1:昆明市延安医院检验科
• 2:中国医学科学院&北京协和医学院医学生物学研究所
• 3:昆明医科大学第三附属医院
• 4:武警云南省总队医院检验科

摘要(Abstract)：

目的:分析人乳头瘤病毒(HPV)在云南地区的感染状况及其分布规律。方法:随机选取云南省昆明、曲靖、昭通、江河、文山及保山6个地区34 401例有性生活史及单一宫颈成年妇女,采集宫颈上皮细胞,运用多重PCR扩增和Luminex x MAP流式荧光杂交分型检测技术对27种HPV进行分型检测,计算HPV在云南省6个地区的感染率并分析HPV的地区差异。结果:共检出HPV阳性标本4 262例,其中保山地区感染率最高(17.4%),曲靖最低(10.8%);各地区感染率两两比较,昭通、曲靖HPV感染率与保山、红河、昆明,保山与文山感染率差异具有统计学意义(P<0.05);单一亚型感染者3 421例(9.9%)、两型混合感染者658例(1.9%)、3型及以上混合感染者184例(0.5%),各地区间差异无统计学意义(P>0.05)。结论:云南省边境地区及经济发达地区HPV感染率较高,HPV单一及混合亚型感染无地区间差异。
Objective: To investigate the infection status and regularities of distribution of HPV in Yunnan province. Methods: 34401 cases of single cervix adult women with sexual life history in Yunnan area were selected randomly to collect cervical epithelial cells. Multiplex PCR amplification and Luminex x MAP flow fluorescent hybridization genotyping technique was used to detect 27 types HPV,and the infection rates of HPV in 6 regions of Yunnan province were calculated and the regional differences of HPV analyzed. Results: A total of 4262 cases were detected as HPV positive. The infection rate was highest in Baoshan( 17. 4%) and lowest in Qujing( 10. 8%). The differences of HPV infection rate between Zhaotong,Qujing and Baoshan,Honghe,and Kunming were statistically significant( P < 0. 05); and the differences of HPV infection rate between Baoshan and Wenshan were statistically significant( P < 0. 05). There were 3421 cases of single subtype infection( 9. 9%),658 cases of mixed infection of two subtypes( 1. 9%),and 184 cases of mixed infection of three subtypes or above( 0. 5%). There was no significant difference between the different regions( P > 0. 05). Conclusion:The HPV infection rate was high in the border areas and the developed areas of Yunnan province,and there was no difference between regions in HPV single subtype and mixed subtypes infection.

关键词(KeyWords)： 基因,结构,病毒;乳头状瘤病毒,人;宫颈肿瘤;流行病学;基因芯片
genes,structural,viral;human papillomaviruses;cervical neoplasm;epidemiology;gene chip

Abstract:

Keywords:

基金项目(Foundation): 国家自然基金项目(81573206,31270030);; 云南省应用基础研究重点项目(2016FA034);; 协和青年基金(3332015149);; 云南省妇科肿瘤内设研究机构中心(2014NS032)

作者(Author): 李铮,史荔,严志凌,李传印,刘舒媛,姚宇峰,刘峰
LI Zheng,SHI Li,YAN Zhiling,LI Chuanyin,LIU Shuyuan,YAO Yufeng,LIU Feng

DOI: 10.19367/j.cnki.1000-2707.2017.04.002

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