田舍,江建新,张浩,张宏,孙诚谊
TIAN She,JIANG Jianxin,ZHANG Hao,ZHANG Hong,SUN Chengyi

• 1:贵州医科大学附院

摘要(Abstract)：

目的:分析原发性肺癌患者血清中小分子代谢产物的改变。方法:利用质子核磁共振(1H NMR)检测18例肺癌(病例组)和同期17例正常体健者(对照组)血清中小分子代谢产物α-葡萄糖、不饱和脂肪酸、肌酐、丙氨酸、谷氨酸盐、丙三醇、苏氨酸、异亮氨基酸、低密度脂蛋白、3-羟丁酸、脂类以及极低密度脂蛋白含量,通过主成分分析法比较两组受检者血清中小分子代谢产物含量的变化。结果:1H NMR图谱能明显区分实验组和对照组血清小分子代谢产物,实验组血清中α-葡萄糖、不饱和脂肪酸及肌酐含量较对照组明显增加,丙氨酸、谷氨酸盐、丙三醇、苏氨酸、异亮氨基酸、低密度脂蛋白、3-羟丁酸、脂类及极低密度脂蛋白较对照组明显减少,差异有统计学意义(P<0.05)。结论:原发性肺癌患者血清中小分子代谢产物与正常体健者存在差异,原发性肺癌患者血清丙氨酸、谷氨酸盐、丙三醇、苏氨酸以及异亮氨基酸可能成为原发性肺癌诊断标志物。
Objective: To analyze the change of small and medium molecular metabolites in blood serum in patients with primary pulmonary carcinoma. Methods:1H NMR was adopted to detect the content of small and medium molecular metabolite,including Alpha-glucose,unsaturated fatty acid,creatinine,alanine,glutamate,glycerol,threonine,isoleucine,low density lipoprotein,3-hydroxybutyrate,lipids,and very low density lipoprotein,in 18 blood specimens from patients with primary pulmonary carcinoma( experimental group) and 17 specimens from healthy controls( control group). The changes of serum small and medium molecular metabolites in the two groups were analyzed and compared by Partial Least Squares( PLS). Results: MR1 H map can clearly distinguish between experimental group and control group in terms of serum small molecule metabolites. In the experimental group,the serum alpha-glucose,unsaturated fatty acid and creatinine content increased significantly compared with those in control group,while alanine,glutamate,glycerol,threonine,isoleucine,low density lipoprotein,3-hydroxybutyrate,lipids and very low density lipoprotein decreased significantly compared with those in control group, the differences were statistically significant( P < 0. 05).Conclusion: There exist differences in serum of small molecular metabolites between patients with primary pulmonary carcinoma and normal healthy people. In patients with primary pulmonary carcinoma,serum alanine,glutamate,glycerol,threonine,isoleucine may be the biomarkers in the diagnosis of primary pulmonary carcinoma.

关键词(KeyWords)： 肺肿瘤;代谢组学;质子核磁共振;诊断;生物学标志物
lung cancer;metabonomics;proton nuclear magnetic resonance;diagnosis;biomarkers

Abstract:

Keywords:

基金项目(Foundation): 贵州省卫生计生委科学技术基金项目(gzwjkj2014-1-004);; 贵阳市科技计划项目[(2010)筑科农合字第1-社-19号]

作者(Author): 田舍,江建新,张浩,张宏,孙诚谊
TIAN She,JIANG Jianxin,ZHANG Hao,ZHANG Hong,SUN Chengyi

DOI: 10.19367/j.cnki.1000-2707.2016.08.012

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