CCK-8法检测重组人白细胞介素-2的活性Determination of Recombinant Human Interleukin-2 Activity with Cell Counting Kit-8
马静,路琛,左丽,胡平生,赵星
MA Jing1,LU Chen1,ZUO Li2,HU Pingsheng3,ZHAO Xing2 (1.Department of Biotechnology
摘要(Abstract)：
目的:建立快速、简便、稳定的检测白细胞介素-2(Interleukin-2,IL-2)活性的方法。方法:利用CCK-8(cell counting kit-8)试剂盒,检测3个生产厂家的IL-2对CTLL-2细胞株增殖的影响。结果:IL-2浓度在2～2 000 U/ml范围内,IL-2浓度与细胞增殖成正比,3个厂家的IL-2活性存在差异。结论:CCK-8法简便易行,结果可靠,重复性好,可用于IL-2活性的检测。
Objective: To establish a rapid,convenient and stable method for detecting interleukin-2(IL-2) activity.Methods: IL-2 activity was detected by testing the proliferation of IL-2 stimulation depended CTLL-2 cell strain with cell counting kit-8(CCK-8).Results: When IL-2 concentration was in the range of 2-2000U/ml,the proliferation of CTLL-2 cells was positively correlated with IL-2 concentration.But IL-2 activities from 3 different companies were different.Conclusions: Method with CCK-8 is convenient,reliable and reproducible for detecting IL-2 activity.
关键词(KeyWords)：
白细胞介素2;CCK-8;生物技术
interleukin-2;cell counting kit-8;biotechnology
基金项目(Foundation):
作者(Author):
马静,路琛,左丽,胡平生,赵星
MA Jing1,LU Chen1,ZUO Li2,HU Pingsheng3,ZHAO Xing2 (1.Department of Biotechnology
DOI: 10.19367/j.cnki.1000-2707.2011.05.019
参考文献(References)：
- [1]刘佳,单安山,孙进华.白细胞介素-2的研究进展与应用[J].黑龙江畜牧兽医,2009(11):19-20.
- [2]国家药典委员会.中华人民共和国药典[S].2部.北京:化学工业出版社,2005:附录57-58.
- [3]张银霞.CTLL-2细胞株的饲养[J].陕西医学检验,1998(11):13-14.
- [4]弗雷谢尼RI.动物细胞培养[M].4版.北京:科学出版社,2004:363-366.
- [5]侯春梅,李新颖,叶伟亮,等.MTT法和CCK8法检测悬浮细胞增殖的比较[J].军事医学科学院院刊,2009(8):400-401.
- [6]李红艳,夏启胜,徐梅,等.MTT、MTS、WET-1在细胞增殖检测中最佳实验条件的研究[J],中国康复医学杂志,2005(11):824-826.
- [7]陈巧英,李大金.CD4+CD25+调节性T细胞的生物学特性及功能[J].中国免疫学,2007:780-781.
文章评论(Comment)：
|
||||||||||||||||||
|
||||||||||||||||||