黄勇攀,刘丙进,喻南慧,刘鲜林,孔祥林,罗俊
HUANG Yongpan1,LIU Bingjin1,YU Nanhui1,LIU Xianlin2,KONG Xianglin2,LUO Jun1(1.Department of Pharmacology

• 1:贵阳医学院药理学教研室
• 2:贵阳医学院
• 3:贵阳医学院电镜室

摘要(Abstract)：

目的:观察灵芝三萜类化合物(GLT)对AD模型大鼠海马组织形态的影响。方法:70只大鼠随机分为生理盐水组,假手术组,模型组,溶媒组,GLT低、中、高剂量组;除生理盐水组和假手术组外,其余各组大鼠单侧海马内一次性注射Aβ25-35制作AD大鼠模型,术后生理盐水组、假手术组、模型组大鼠分别灌胃生理盐水,溶媒组灌胃食用油,GLT各组分别灌胃低、中、高剂量的GLT20d,然后采用透射电镜观察海马神经元的超微结构变化。结果:GLT能明显改善模型大鼠脑组织海马CA1区神经元的退行性变化。结论:GLT能改善大鼠海马CA1区神经元的退行性变化。
Objective:To observe the protective effects of triterpenoids from Ganoderma lucidum (GLT) on the modality of hippocampus tissue of Alzheimer disease (AD) model rats.Methods:AD rats induced by injection of Aβ25-35 into one side of hippocampus were treated with GLT orally for 20 days and then ultrastructural changes of neurons were observed under transmission electron microscope.Results:The modality of hippocampus CA1 region neurons of GLT treated AD rats was distinctly improved when compared with that of the model rats that did not receive GLT treatment.Conclusion:GLT can improve recessively changed neurons in hippocampus CA1 region.

关键词(KeyWords)： 灵芝属;阿尔茨海默病;海马;丙二醛;显微镜检查,电子,扫锚透射;大鼠,Sprague-Dawley
Gardenia; Alzheimer disease; hippocampus; malondialdehyde; microscopy,electron,scaning transmission; rats,sprague-dawley

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技攻关项目[D2004-4]

作者(Author): 黄勇攀,刘丙进,喻南慧,刘鲜林,孔祥林,罗俊
HUANG Yongpan1,LIU Bingjin1,YU Nanhui1,LIU Xianlin2,KONG Xianglin2,LUO Jun1(1.Department of Pharmacology

DOI: 10.19367/j.cnki.1000-2707.2008.06.026

参考文献(References)：

• [1]Wang G,Zhang J,Mizuno T,et a1.Antitumor active poly-saccharides from the Chinese mushroom Songshan lingzhi,the fruiting body of Ganoderma tsugae[J].Biosci Biotech-nol Biochem,1993(6):894-900.
• [2]Sharma M,Gupta YK.Chronic treatment with trans resver-atml prevents intracerebmventricular streptozotocin in-duced cognitive impairment and oxidative stress in rats[J].Life Sciences,2002(71):2489-2498.
• [3]Ling YW,Yuan H,Li PCH.Changes of NOS positive cells and astrocytes in rats with memory deficit induced byβ-amyloid peptide fragment25-35[J].Chinese Journal of His-tochemistry and Cytochemistry,2003(1):36-41.
• [4]郭燕君,袁华,张俐娜,等.灵芝多糖对阿尔茨海默病大鼠海马组织形态学及抗氧化能力的影响[J].解剖学报,2006(10):509-513.
• [5]Cong ZH,Li JR,Wang YF,et a1.The discussion of re-search of Ganoderma Lucidum and the therapy principle of Chinese traditional medicine[J].Medical Journal of Bei-jing Medicine College,1981(1):6-9.
• [6]罗俊,林志彬.灵芝三萜类化合物药理作用研究进展[J].药学学报,2002(7):574-578.
• [7]Liu JL.Pharmacy esearch overviewof Ganoderma lucidum polysaccharide[J].Guangdong Pharmacy,2000(2):46-48.

文章评论(Comment)：