庄景义,彭吾训
ZHUANG Jingyi,PENG Wuxun

• 1:贵州省肿瘤医院急诊科
• 2:贵阳医学院附院急诊创伤外科

摘要(Abstract)：

目的:观察吸附有骨形态发生蛋白(BMP)及碱性成纤维细胞生长因子(bFGF)的双相陶瓷生物骨(BCBB)植入兔股部肌袋内的成骨过程,评价BCBB/BMP/bFGF的成骨作用。方法:成年新西兰大白兔60只,随机分为4组;A组股部肌袋内植入BCBB/BMP/bFGF,B组植入BCBB/BMP,C组植入BCBB/bFGF,D组植入BCBB;分别于术后4、8和12周取材,每个时间点每组取5例标本进行外观及组织切片观察,并检测钙含量及钙磷比。结果:外观见各时间点标本A组较B组、C组和D组色泽红润,与周围组织连接紧密,组织切片术后各时间点新骨形成面积比较,A组大于B组、C组和D组,B组大于C组和D组,差异具有统计学意义(P<0.05),C组、D组间差异无统计学意义(P>0.05);X射线能谱分析,术后12周时A组钙含量及钙磷比大于B组、C组和D组,差异具有统计学意义(P<0.05)。结论:BCBB是生长因子的良好吸附载体,且吸附有BMP及bFGF的BCBB具有较强的成骨能力,bFGF能够促进BMP的诱导成骨作用。
Objective: To observe the osteogenesis of biphasic ceramic biologic bone(BCBB) absorbing bone morphogenetic protein(BMP) and basic fibroblast growth factor( bFGF) implanted into muscle bag of rabbit thigh,and to evaluate the osteogenic effect of BCBB /BMP /bFGF. Methods: Sixty rabbits were randomly divided into four groups:group A,B,C and D implanted with BCBB /BMP /bFGF,BCBB /BMP,BCBB /bFGF and BCBB into muscle bag of rabbit thigh respectively. The specimens were harvested separately in 4,8 and 12 weeks after operation. 5 samples were taken at each time point in each group. Appearance and histological observation were carried out,the calcium content and the ratio of calcium and phosphorus were detected. Results: The appearance of the samples in group A was redder than those of groups B,C and D,and closely connected with surrounding tissue.Histological observation found that new bone areas of the samples in group A were larger than those of groups B,C and D,and those of group B were larger than in groups C and D( P < 0. 05),and the difference between group C and D was not significant( P > 0. 05). X-ray energy spectrum analysis(EDAX)showed that the calcium content was more and the ratio of calcium and phosphorus of group A was higher than those of group B,C and D in 12 week after operation,and the differences were obvious(P < 0. 05). Conclusions: BCBB is a fine absorption carrier of growth factors,and BCBB absorbed BMP and bFGF has strong osteogenic activity,and the osteogenesis effect of BMP can be promoted by bFGF.

关键词(KeyWords)： 双相陶瓷生物骨;骨形态发生蛋白质类;成纤维细胞生长因子;组织工程
biphasic ceramic biologic bone;bone morphogenetic proteins;basic fibroblast growth factor;tissue engineering

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅贵阳医学院社发联合基金项目[黔科合(2010)3165];; 贵州省科学技术基金项目[黔科合J字(2013)2038号];; 贵州省卫生厅科技基金项目(gzwkj2010-1-039)

作者(Author): 庄景义,彭吾训
ZHUANG Jingyi,PENG Wuxun

DOI: 10.19367/j.cnki.1000-2707.2014.03.011

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