苏荣华,刘颖,吕明庄,王光义
SU Ronghua,LIU Ying,LV Mingzhuang,WANG Guangyi

• 1:贵州医科大学
• 2:贵州医科大学附院针灸科

摘要(Abstract)：

目的:观察太乙神针对类风湿性关节炎(RA)大鼠肿瘤坏死因子-ɑ(TNF-ɑ)、可溶性CD4(s CD4)和可溶性CD8(s CD8)的影响。方法:选取健康SD大鼠40只,随机抽取10只为正常对照组(右侧后足垫皮下注射生理盐水),余30只采用弗氏完全佐剂建立类风湿性关节炎大鼠模型(RA),造模成功后,随机分为模型组(捉拿但不治疗)、普通艾灸组(普通艾条治疗)和太乙神针灸组(太乙神灸治疗),治疗结束后,检测各组大鼠足肿胀度及血清中TNF-ɑ,s CD4和s CD8的含量。结果:模型组大鼠足肿胀度及血清中TNF-ɑ、s CD4和s CD8的含量较正常对照组明显升高,差异有统计学意义(P<0.05);普通艾灸组和太乙神针灸组较模型组大鼠足肿胀度及血清中TNF-ɑ、s CD4和s CD8的含量明显降低,差异有统计学意义(P<0.05),其中太乙神针灸组效果尤为明显(P<0.05)。结论:太乙神针能减轻RA大鼠足部肿胀度,降低血清中TNF-a、s CD4和s CD8含量,这可能是太乙神针治疗类风湿性关节炎的内在机制之一。
Objective: To investigate the effect of Taiyi Shenzhen on TNF-α and soluble CD4,CD8 of rheumatoid arthritis rats. Methods: Selecting 40 SD rats,randomly choosing 10 as the control group( subcutaneous injection normal saline in right hind legs),adopting Freund's complete adjuvant to establish RA rats model for the rest 30 rats,then further dividing the 30 rats in to model group( Nonetreatment),normal moxibustion group( treated with normal moxibustion) and Taiyi Shenzhen group( treated with Taiyi Shenzhen). After treatment,testing feet degree of swelling of rats and content of TNF-α,s CD4 and s CD8 in serum. Results: Feet degree of swelling,content of TNF-α,s CD4 and s CD8 in serum of Model group were significantly higher than control group,differences were statistical significant( P < 0. 05); feet degree of swelling,content of TNF-α,s CD4 and s CD8 in serum of normal moxibustion group and Taiyi Shenzhen group showed lower feet degree of swelling,content of TNF-α,s CD4 and s CD8 in serum than model group,differences were statistical significant( P < 0. 05),among which Taiyi Shenzhen group showed most obvious treatment effect( P < 0. 05). Conclusion: Taiyi Shenzhen could greatly reduce feet swelling,decrease content of TNF-α,s CD4 and s CD8 in serum. This might be one of the mechanisms of the treatment of Taiyi Shenzhen on RA.

关键词(KeyWords)： 类风湿性关节炎;肿瘤坏死因子-α;可溶性CD4;可溶性CD8;大鼠;太乙神针
rheumatoid arthritis;tumor necrosis factor-α;soluble CD4;soluble CD8;rats;Taiyi Shenzhen

Abstract:

Keywords:

基金项目(Foundation): 国家中管局“全国中医药名老专家传承工作室建设项目”(2014)

作者(Author): 苏荣华,刘颖,吕明庄,王光义
SU Ronghua,LIU Ying,LV Mingzhuang,WANG Guangyi

DOI: 10.19367/j.cnki.1000-2707.2015.10.008

参考文献(References)：

• [1]叶任高,陆再英.内科学[M].5版.北京:人民卫生出版社,2002:897-903.
• [2]匡建军.太乙神针配活痹饮治疗类风湿性关节炎25例[J].湖南中医杂志,1995(5):38.
• [3]黄亏炎,黄禹.太乙雷火神针治疗类风湿性关节炎60例[J].中国民间疗法,2008(8):17-18.
• [4]陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1993:9821.
• [5]耿昊,李志刚.思考太乙神针[J].四川中医,2006(6):109-111.
• [6]李守中.中医药对类风湿关节炎TNF-ɑ,IL-6影响的研究进展[J].内蒙古中医药,2014(26):83-84.
• [7]胡颖,宋作珪.自身免疫性疾病患者血清可溶性CD4、CD8的测定及临床意义[J].浙江医学,2003(12):719-720,735.

文章评论(Comment)：