高娜,衡燮,姬秋彦,梁疆莉,顾琴,马艳,史荔,孙明波
GAO Na,HENG Xie,JI Qiuyan,LIANG Jiangli,GU Qin,MA Yan,SHI Li,SUN Mingbo

• 1:中国医学科学院&北京协和医学院医学生物学研究所

摘要(Abstract)：

目的:探讨培养基、接种菌量与pH值对破伤风梭状芽孢杆菌产毒的影响。方法:采用示胨三号、示胨二号、胃蛋白胨及自制肉汤4种培养基培养破伤风梭状芽孢杆菌,比较毒素产量;选择最优培养基,按照培养基体积的1%,2%,3%,4%接种破伤风梭状芽孢杆菌,培养160 h,选取合适接种菌量;使用最优培养基与接种量,分别用初始pH 7. 5、7. 2、7. 0的3种培养基培养破伤风梭状芽孢杆菌,比较毒素产量。结果:示胨三号产毒量优于其他3种蛋白胨,差异具有统计学意义(P <0. 05);接种菌量大于2%时产毒量最优,3种初始pH培养条件对产毒量无明显影响(P> 0. 05)。结论:初步筛选出最优破伤风毒素培养基及接种量,为破伤风疫苗工艺研究及扩大化生产提供依据。
Objective: To investigate effect of culture medium,inoculum amount and pH Value on the toxin production of Clostridium Tetanus. Methods: Comparative analysis was performed using three types of medium: peptone 3,peptone 2 and stomach peptone,together with lab-made solution as culture medium to cultivate Clostridium Tetanus to compare toxin production; then selecting rime culture medium: inoculate Clostridium Tetanus according to 1%,2%,3%,4% of culture medium size respectively for 160 h,then selecting inoculum ratio. In the meantime,using the best culture medium and inoculum amount to cultivate Clostridium Tetanus with initial pH value of 7. 5,7. 2 and 7. 0 to analyze the toxin production. Results: Peptone 3 showed significantly better results than the other two peptones,difference was statistically significant(P < 0. 05). When the inoculation amount was greater than 2%,toxin production could produce the optimal yield. There was no significant difference between the three initial pH levels,it has no effect on the final amount of toxin production(P < 0. 05).Conclusion: The experiment confirmed the cultivation process of inoculum amount and pH value of tetanus toxin,and provide guarantee for the research and expansion of tetanus vaccine technology.

关键词(KeyWords)： 破伤风梭状芽孢杆菌;类毒素;培养基;培养技术;接种菌量
Clostridium tetanus;toxoid;culture;culture technology;amount of inoculum

Abstract:

Keywords:

基金项目(Foundation): 重大新药创制科技重大专项基金资助项目(2015ZX09101031);; 中国医学科学院医学与健康科技创新工程资助项目(2016-I2M-1-019)

作者(Author): 高娜,衡燮,姬秋彦,梁疆莉,顾琴,马艳,史荔,孙明波
GAO Na,HENG Xie,JI Qiuyan,LIANG Jiangli,GU Qin,MA Yan,SHI Li,SUN Mingbo

DOI: 10.19367/j.cnki.1000-2707.2018.10.022

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