王和,徐英
Wang He, et al Department of Microbiol

• 1: 贵阳医学院微生物学教研室
• 2:贵阳医学院微生物学教研室

摘要(Abstract)：

绿脓杆菌L型可在非高渗透性无抗生素的1640液中传代培养。其形态为圆球或长丝状,丧失鞭毛,不运动,可通过0.3μm孔径的滤菌器,酶活性和对抗生素的敏感性不同于其亲代细菌,并可进入HeLa细胞内和致细胞病变。
L forms of pseudomonas aeruginosa could be subcultured in non-high osmotic 1640 liquid medium without antibiotic. They were spheroid an d elongate bodies, lost the flagellum and could not move. The L forms were passable to filters of 0.3μm pore size and different from the parent bacteria from which they were derived on the activities of enzymes and the susceptibility to antibiotics. The L forms of P. aeruginosa were within HeLa cells and cytopathic on cultures of the cells.

关键词(KeyWords)： 绿脓杆菌;L型;培养基
pseudomonas aeruginosa;L form;culture media

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 王和,徐英
Wang He, et al Department of Microbiol

DOI: 10.19367/j.cnki.1000-2707.1990.01.019

参考文献(References)：

• 1． 余.医学微生物学.北京:人民卫生出版社,1983:346～360．
• 2． 王和.L型鸡蛋培养基的制备和细菌L型的诱导.贵阳医学院学报 1988;13(1) :42．
• 3． 王和.L型细菌培养特性的观察.贵阳医学院学报 1989;14(2) :111．
• 4． 杨履渭.微生物学及检验技术.广州:广东科技出版社,1986;440～445．

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