贵州医科大学学报

2018, v.43;No.218(11) 1256-1262

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氧化苦参碱磷脂复合物纳米结构脂质载体的制备
Preparation of Nanostructured Lipid Carrier of Oxymatrine Phospholipid Complex

张科;严俊丽;李婉蓉;贺智勇;姜丰;周雪;吴林菁;甘诗泉;明先彦;沈祥春;陶玲;
ZHANG Ke;YAN Junli;LI Wanrong;HE Zhiyong;JIANG Feng;ZHOU Xue;WU Linjing;GAN Shiquan;MING Xianyan;SHEN Xiangchun;TAO Ling;Department of Parasitology in Basic Medical College,Key Laboratory of Modern Pathogenic Biology,Guizhou Medical University;State Key Laboratory on Efficacy and Utilization of Medicinal Plants,Guizhou Engineering Center for Efficient Utilization of Natural Drug Resources,High Educational Key Laboratory of Natural Medicinal Pharmacology and Drug Ability,Guizhou Medical Universit

摘要(Abstract):

目的:采用微乳法制备氧化苦参碱磷脂复合物纳米结构脂质载体(OMT-PC-NLC)。方法:通过伪三元相图考察OMT-PC-NLC初乳处方,再将初乳分散于冰水中固化,以包封率为指标,考察固化时间、外水相体积、搅拌速度对OMT-PC-NLC制备工艺的影响。结果:以硬脂酸为固态脂质,油酸为液态脂质,吐温/泊洛沙姆组成的复合乳化剂比例为2∶1,乙醇为助乳化剂,乳化剂与助乳化剂的比例(Km)为1∶1,油相Km为1∶9,固化时间为10 min,外水相体积为175 m L,将初乳在1 750 r/min搅拌状态下分散于冰水中、固化,制备得到OMT-PC-NLC平均粒径为315 nm,粒径范围为161~650 nm。结论:采用微乳法成功制备OMT-PC-NLC,制备工艺简单、操作方便。
Objective: To prepare the nanostructured lipid carrier of oxymatrine phospholipid complex(OMT-PC-NLC) by microemulsion method. Methods: The prescription of OMT-PC-NLC colostrum was investigated by pseudo-ternary phase diagram; the colostrum was dispersed in ice water to solidify,and the encapsulation efficiency was taken as the index. The effects of curing time,volume of external water phase and stirring speed on the preparation process of OMT-PC-NLC were investigated.Results: Using stearic acid as solid lipid and oleic acid as liquid lipid,the ratio of Tween/Poloxamer was 2∶ 1. The ratio of ethanol as co-emulsifier,the ratio of emulsifier to co-emulsifier(Km) was 1∶ 1;the oil phase Km was 1∶ 9; Curing time was 10 mins; the volume of external water phase was 175 m L.Colostrum was dispersed in ice water at 1 750 r/min and solidified. The average particle size of OMTPC-NLC was 315 nm and the particle size range was 161 ~ 650 nm. Conclusion: The preparation of OMT-PC-NLC by microemulsion method is simple and easy to operate.

关键词(KeyWords): 氧化苦参碱;微乳法;纳米结构脂质载体;包封率;高效液相色谱法
oxymatrine;micro-emulsion method;Nanostructured lipid carrie;encapsulation efficiency;high performance liquid chromatography

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基金项目(Foundation): 国家自然科学基金项目(81860752);; 贵州省高层次创新型人才百层次人才项目[贵州科技厅黔科合人才(2015)4029号];; 贵州医科大学药学国际科技合作基地项目[黔科合平台人才(2017)5802];; 贵州省科学技术基金重点项目[黔科合JZ字(2015)2002号];; 黔南州科技计划项目[黔南科合社字(2017)74号];; 遵义医学院基础药理省部共建教育部重点实验室项目[黔教合KY字(2017)379];; 贵阳医学院博士启动基金项目[院博合J字(2017-24)号];; 贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2018-086);; 贵州省教育厅青年科技人才成长项目[黔教合KY字(2018)181]

作者(Author): 张科;严俊丽;李婉蓉;贺智勇;姜丰;周雪;吴林菁;甘诗泉;明先彦;沈祥春;陶玲;
ZHANG Ke;YAN Junli;LI Wanrong;HE Zhiyong;JIANG Feng;ZHOU Xue;WU Linjing;GAN Shiquan;MING Xianyan;SHEN Xiangchun;TAO Ling;Department of Parasitology in Basic Medical College,Key Laboratory of Modern Pathogenic Biology,Guizhou Medical University;State Key Laboratory on Efficacy and Utilization of Medicinal Plants,Guizhou Engineering Center for Efficient Utilization of Natural Drug Resources,High Educational Key Laboratory of Natural Medicinal Pharmacology and Drug Ability,Guizhou Medical Universit

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DOI: 10.19367/j.cnki.1000-2707.2018.11.004

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