贵州医科大学学报

2018, v.43;No.217(10) 1217-1220+1231

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结肠癌组织中BLM、RECQ4的表达水平及临床意义
Expression Levels of BLM and RECQ4 in Colon Cancer Tissues and Their Clinical Significance

郑艺;刘杰麟;韩莹;冯江龙;
ZHENG Yi;LIU Jielin;HAN Ying;FENG Jianglong;Department of Immunology,School of Basic Medicine,Guizhou Medical University;Department of Pathology,School of Basic Medicine,Guizhou Medical University;

摘要(Abstract):

目的:检测结肠癌组织中BLM解旋酶和RECQ4解旋酶的表达水平,分析其与结肠癌临床病理特征的关系。方法:选取手术切除的结肠癌组织及其癌旁结肠组织标本54对,采用免疫组织化学染色的方法检测结肠癌组织及癌旁组织中BLM及RECQ4蛋白的表达,比较不同TNM临床分期和分化程度的结肠癌患者癌组织中BLM和RECQ4蛋白水平。结果:BLM和RECQ4蛋白在结肠癌及癌旁组织中均有表达,但在癌组织中BLM和RECQ4蛋白表达水平高于癌旁组织(P <0. 05); BLM和RECQ4蛋白在Ⅲ期结肠癌组织中的表达水平高于Ⅱ期和Ⅰ期结肠癌组织(P <0. 05),低分化结肠癌组织中BLM和RECQ4蛋白的表达水平高于中分化和高分化结肠癌组织(P <0. 05)。结论:BLM和RECQ4在结肠癌组织中高表达,且与TNM临床分期和组织病理学分型相关,可作为结肠癌诊断和预后判断的指标。
Objective: To detect expression levels of BLM helicase and RECQ4 helicase in colon cancer tissues,and analyze its relationship with clinicopathological features of colon cancer. Methods:Specimens of Surgically resected colon cancer tissues and their para-cancerous tissues were selected from 54 patients. The expression of BLM and RECQ4 protein in colon cancer tissues and their paracancerous tissues were observed by immunohistochemistry. BLM and RECQ4 protein levels in cancer tissues were compared by different clinical and differentiated TNM stages. Results: BLM and RECQ4 were expressed in colon cancer tissues and their para-cancerous tissues,but protein expression levels are higher in cancer tissues than in para-cancerous tissues(P < 0. 05). The expression levels of BLM and RECQ4 proteins at stage III colon cancer tissues were higher than those at stage II and stage I(P< 0. 05). The expression levels of BLM and RECQ4 proteins in poorly-differentiated colon cancer tissues were higher than those in moderately-differentiated and well-differentiated tissues(P < 0. 05).Conclusion: BLM and RECQ4 were highly expressed in colon cancer tissues and were associated with clinical TNM stages and tumor differentiation,which can be used as indexes for colon cancer diagnosis and prognosis.

关键词(KeyWords): RecQ解旋酶类;结肠肿瘤;癌;肿瘤分期;免疫组织化学染色
RECQ helicase;colon tumor;cancer;tumor staging;immunohistochemical staining

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81360349);; 贵州省应用基础研究计划重大专项子课题资助项目[黔科合J重大字(2015)2003]

作者(Author): 郑艺;刘杰麟;韩莹;冯江龙;
ZHENG Yi;LIU Jielin;HAN Ying;FENG Jianglong;Department of Immunology,School of Basic Medicine,Guizhou Medical University;Department of Pathology,School of Basic Medicine,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2018.10.018

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