张明昶,李健,张小勇
ZHANG Mimg chang, LI Jian, ZHANG Xiao yong (Department of Pharmacy, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China)

• 1:贵阳中医学院药学系
• 2:贵阳中医学院药学系
• 3:贵阳中医学院药学系 贵州贵阳550002
• 4:贵州贵阳550002

摘要(Abstract)：

目的 :测定知柏地黄丸中盐酸小檗碱的含量。方法 :根据在紫外光的激发下盐酸小檗碱可产生荧光的性质 ,应用双波长薄层扫描法 ,选择λS=342nm ,λR=2 6 2nm测定知柏地黄丸中盐酸小檗碱。结果 :测定了三个批号的产品 ,其含量在 0 0 14 86 %～ 0 0 194 8%之间。结论 :此法操作简便 ,结果可靠。加样回收率为99 0 1% ,RSD为 2 2 %。
Objective:To analyse the content of berberine hydrochloride in Zhibai Dihuang Wan.Methods:According to the fact that berberine hydrochloride can produce fluorescence under the excitation of ultraviolet light, a quantitative method was developed for determination of berberine hydrochloride by the scanning method of two-wavelengths thin layer chromatography. The λS was 342nm and the λR 262nm.Results: Three sets of product were determined. Their contents of berberine hydrochloride were in the range of 0 01486～0 01948%. The recovery rate and the relative standard deviation were 99 01% and 2 2% respectively.Conclusion:This method is easy to operate and the results should be consistant.

关键词(KeyWords)： 知柏地黄丸;小檗碱;吸收测定法,光子
Zhibai Dihuang Wan; berberine hydrochloride;absorptionetry,photon

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张明昶,李健,张小勇
ZHANG Mimg chang, LI Jian, ZHANG Xiao yong (Department of Pharmacy, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China)

DOI: 10.19367/j.cnki.1000-2707.2002.06.016

参考文献(References)：

• [1]梁生旺,刘伟.中药制剂定量分析[M ].北京:中国中医药出版社,1997.149.
• [2]阚毓铬.中药制剂分析[M ].南京:南京大学出版社.1992.258.
• [3]国家药典委员会.中华人民共和国药典[S].北京:化学工业出版社,2000.一部附录VIB .37-38.

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