归芍胶囊中白芍的鉴别和芍药苷含量测定Identification of Radix paeoniae alba in Guishao Capsules with TLC and Determination of Paeoniflorin Content with HPLC
樊燕,饶光玲
FAN Yan1,RAO Guanglin2(1.Nursing Vocational College of Guiyang City
摘要(Abstract)：
目的:鉴别归芍胶囊中的白芍和测定归芍胶囊中芍药苷的含量。方法:采用薄层色谱法鉴别白芍,高效液相色谱法测定芍药苷的含量,色谱柱为Shimadzu VP-ODS(4.6 mm×150 mm,5μm),以乙腈-0.1%磷酸溶液(14∶86)为流动相、检测波长230 nm、柱温35℃。结果:归芍胶囊中白芍的鉴别方法和芍药苷的含量测定方法专属性强,在40～320 mg/L内线性关系良好,平均回收率为99.94%(RSD=1.15%)。结论:薄层色谱法鉴别归芍胶囊中白芍,HPLC法测定芍药苷含量的方法简便、灵敏、准确。
Objective: To identify Radix paeoniae alba and to determin paeoniflorin content in Guishao capsules.Methods: Radix paeoniae alba was identified with thin layer chromatography(TLC).Paeoniflorin content was determined with high performance liquid chromatography(HPLC).The HPLC method was carried out on Shimadzu VP-ODS(4.6 mm×150 mm,5 μm)column using acetonitrile-0.1% phosphoric acid(14∶86) as mobile phase Detection wavelength was 230nm,and temperature of column was 35℃.Results: The identification method and the content determination method showed strong specificity.The calibration curve for paeoniflorin was linear in the range of 40~320 μg/ml.The average recovery was 99.94%,and RSD was 1.15%.Conclusions: The methods are simple,sensitive and accurate.
关键词(KeyWords)：
色谱法,薄层;色谱法,高压液相;白芍;芍药
chromatography,thin layer;chromatography,high pressure liquid;radix paeonta veichii
基金项目(Foundation): 贵州省中药现代化科技产业研究开发项目[黔科合中药专字(2003)74]
作者(Author):
樊燕,饶光玲
FAN Yan1,RAO Guanglin2(1.Nursing Vocational College of Guiyang City
DOI: 10.19367/j.cnki.1000-2707.2012.03.013
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