黄芪不同提取部分抗Ⅰ型和Ⅱ型单纯疱疹病毒的实验研究The Efficacies of Various Parts of Astragalus Membranaceus Against Herpes Simplex Virus(HSV) in Vitro
孙晓娟,董熙昌
Sun Xiaojuan, et al Department of Microbiology
摘要(Abstract)：
本文报道8种黄芪提取部分即AⅠ、AⅢ,AⅣ、AⅦ、AⅧ、AⅨ、AⅩ在VeroE_6细胞中对Ⅰ型和Ⅱ型单纯疱疹病毒(HSV-1和HSV-2)的作用。细胞病变(CPE)抑制试验结果表明,AⅠ、AⅥ和AⅦ对HSV-1有抑制作用,AⅠ和AⅥ对HSV-2有抑制作用。AⅥ在体外不能直接灭活HSV-1,但能抑制已感染细胞的病毒复制。
The effects of eight parts of Astragalus membranaceus (AⅠ, AⅢ, AⅣ, AⅥ, AⅦ, AⅧ, AⅨ, and AⅩ) against HSV-1 and HSV-2 were studied by cytopathic effect (CPE) inhibition assay. The AⅢ, AⅣ, AⅧ, AⅨ, and AⅩ did not show any inhibitory effect on HSV-1 and HSV-2, while the AⅠ AⅥ, and AⅦ showed inhibition to HSV-1. When 10 TCID_(50) viruses were used, the minimum inhibitory concentrations (MIC) of AⅠ AⅥ, and AⅦ against HSV-1 were 1.0, 0.312, and 0.250 mg/ml respectively, while the MIC of AⅠ and AⅥ against HSV-2 were 1.0 and 0.416 mg/ml. When the amount of viruses increased to 100 TCID_(50), the MIC of AⅠ, AⅥ, and AⅦ against HSV-1 were 1. 667, 0.625, and 0.50 mg/ml respectively, while the MIC of AⅠ and AⅥ against HSV-2 were 2.0 and 0.625 mg/ml. AⅥ could not inactivate HSV-1 directly in vitro. However, AⅥ could inhibite viral replication after virus invaded in cells and no CPE could be seen under microscope.
关键词(KeyWords)：
黄芪;人疱疹病毒;细胞病变抑制试验
Astragalus membranaceus; herpesvirus hominis; cytopathic effect inhibition assay
基金项目(Foundation):
作者(Author):
孙晓娟,董熙昌
Sun Xiaojuan, et al Department of Microbiology
DOI: 10.19367/j.cnki.1000-2707.1991.04.007
参考文献(References)：
- 1． 中国科学院上海药物研究所.黄芪多糖的免疫促进作用.科学通报1979;16:764．
- 2． 候云德,等.黄芪某些生物学活性的有效部分的研究.中西医结合杂志1984;4(7) :420～424．
- 3． G D华斯.动物组织培养技术.北京:科技出版社,1982:59．
- 4． 贵阳医学院微生物学教研室.医学微生物学实验指导.第三册.贵阳:贵阳医学院,1985．
- 5． Moore DF, et al. Virus inhibition assay for measurement of acyclovir levels in human plasma and urine. Antimicrob Agents Chemother 1981; 20 (6) : 787-792．
- 6． 陈瑞琪,等.紫草多糖在组织培养管内抑制单纯疱疹病毒的实验研究.江西医学院学报1982;2:5～9．
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