王珍珍,张景照,许乾丽,茅向军,郑丽会,夏玉吉
WANG Zhenzhen1,ZHANG Jingzhao1,XU Qianli2,MAO Xiangjun2,ZHENG Lihui3,XIA Yuji3(1.School of Pharmacology

• 1:贵阳医学院药学院
• 2:贵州省食品药品检验所
• 3:贵州师范大学

摘要(Abstract)：

目的:建立开喉剑喷雾剂中八爪金龙主要成分岩白菜素含量的测定方法。方法:采用高效液相色谱法,色谱柱为Hypersil ODS2-C18柱(5μm,200 mm×4.6 mm),流动相为甲醇-水(17∶83),检测波长为275 nm,流速为1.0 ml/min。结果:岩白菜素进样量在0.050 55～1.365 00μg线性关系良好(r=0.999 9),平均回收率为99.5%,RSD为2.1%。结论:该方法简便、准确、重现性好,可用于该制剂中岩白菜素含量的测定。
Objective: To establish a method for content determination of bergenin in Kaihoujian Spray.Methods: The HPLC method was performed on Hypersil ODS2-C18(5 μm,200 mm×4.6 mm) column with methanol-water(17∶83) as mobile phase.Detection wavelength was 275 nm,and the flow rate was 1.0 ml/min.Results: The linear range of bergenin was 0.050 55~1.365 00 μg(r=0.999 9).The mean recovery was 99.5%,and RSD was 2.1%.Conclusions: The method is simple and accurate for determination of bergenin content in this drug.

关键词(KeyWords)： 开喉剑喷雾剂;岩白菜;色谱法,高压液相
kaihoujian spray;bergennia purpurascens;chromatography,high pressure liquid

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 王珍珍,张景照,许乾丽,茅向军,郑丽会,夏玉吉
WANG Zhenzhen1,ZHANG Jingzhao1,XU Qianli2,MAO Xiangjun2,ZHENG Lihui3,XIA Yuji3(1.School of Pharmacology

DOI: 10.19367/j.cnki.1000-2707.2012.03.015

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