孔义华,焦彦朝,刘杰麟,高博,罗阿东,曹云恒
KONG Yihua1,2,JIAO Yanchao1,LIU Jielin2,GAO Bo1,LUO Adong1

• 1:贵州省出入境检验检疫局技术中心
• 2:贵阳医学院医学检验系

摘要(Abstract)：

目的:探讨在食品检验中单核细胞增生性李斯特菌的检验方法。方法:采用常规培养法、全自动酶联免疫荧光分析仪筛选法、基因探针化学发光检测法、实时荧光定量PCR检测法对英国食品检验能力评价体系(FEPAS)牛肉单核细胞增生性李斯特菌水平测试提供的二份盲样(M164d02A和M164d02B)进行检测。结果:4种检测方法检测结果一致,样本M164d02A检出单核细胞增生性李斯特菌、样本M164d02B未检出单核细胞增生性李斯特菌,而实时荧光定量PCR检测法灵敏度较高,检测周期较短、特异性较高。结论:实时荧光定量PCR检测法在单核细胞增生性李斯特菌的检测中具有灵敏度高、检测时间短、特异性高等特点。
Objective: To compare several methods for detection of the Listeria monocytogenes in the food.Methods: Four different methods including routine culture method,full-automatic enzyme-linked immunoassay screening method by fluorescence analyzer,gene probe chemiluminescence method(AccuProbe),and real-time fluorescent quantitative PCR method were used to test two blind Listeria monocytogene samples(M164d02A and M164d02B) provided by England food examination performance assessment scheme(FEPAS),and sensitivity,detection time and specificity among the 4 methods were compared.Results: The four testing methods showed the same results that Listeria monocytogenes were detected in sample M164d02A but not detected in sample M164d02B,while Real-time quantitative fluorescence PCR detection method was more sensitive with shorter detection time and higher specificity.Conclusions: Real-time quantitative fluorescence PCR detection method is sensitive with short detection time and high specificity for detection of Listeria monocytogenes.

关键词(KeyWords)： 利斯特菌,单核细胞增生;核酸探针;实时荧光定量PCR;检疫
Listeria monocytogenes;nucleic acid probes;real-time fluorescent quantitative PCR;quanrantine

Abstract:

Keywords:

基金项目(Foundation): 国家质检总局科技计划项目(2009IK165);; 贵州省高层次人才科研条件特助经费项目(TZJF-2007年-18号)

作者(Author): 孔义华,焦彦朝,刘杰麟,高博,罗阿东,曹云恒
KONG Yihua1,2,JIAO Yanchao1,LIU Jielin2,GAO Bo1,LUO Adong1

DOI: 10.19367/j.cnki.1000-2707.2012.03.007

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