贵州医科大学学报

2018, v.43;No.217(10) 1154-1158+1168

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慢性氟中毒大鼠学习记忆能力与血清、海马及皮质脑组织中PGC-1α水平的相关性
Correlation Study on the PGC-1α Level in Cortex Tissue and Hippocampus,Serum with Learning and Memory in the Brain of Rats with Chronic Fluorosis

刘仙红;陈丹;曾晓晓;董阳婷;邓婕;齐晓岚;吴昌学;李毅;宋辉;官志忠;
LIU Xianhong;CHEN Dan;ZENG Xiaoxiao;DONG Yangting;DENG Jie;QI Xiaolan;WU Changxue;LI Yi;SONG Hui;GUAN Zhizhong;Key Laboratory of Endemic and Ethnic Group Disease of Ministry of Education,Guizhou Medical University;Key Laboratory of Molecular Biology of Guizhou Province,Guizhou Medical University;Key Laboratory of Molecular Biology,Guizhou Medical University;Department of Pathophysiology,Affiliated Hospital of Guizhou Medical University;

摘要(Abstract):

目的:研究慢性氟中毒大鼠脑组织中过氧化物酶体增殖激活受体γ辅激活因子(PGC-1α)水平,探讨PGC-1α与学习记忆能力的关系。方法:SD大鼠60只,随机分为对照组(饮水含氟量<0. 5 mg/L)、低氟组(饮水含氟量为5. 0 mg/L)、高氟组(饮水含氟量为50. 0 mg/L),染氟时间分别为3个月及6个月;分别于染氟3月及6月时,用Morris水迷宫测定大鼠学习记忆能力,采用氟离子选择电极法测定大鼠血清氟含量,用蛋白印记方法检测大鼠海马及皮质组织中PGC-1α蛋白表达水平,分析大鼠海马及皮质PGC-1α蛋白水平与血清氟含量及空间学习记忆能力的相关性。结果:染氟大鼠逃避潜伏期较对照组显著延长、穿越平台次数及逗留平台象限时间明显缩短,以染氟6月组大鼠更明显(P <0. 05);血清氟含量随着染氟浓度的增高及染氟时间的延长而升高,高氟组大鼠血氟含量显著高于对照组,以染氟6月组大鼠尤为明显(P <0. 05);染氟大鼠海马区和皮质区的PGC-1α蛋白表达水平随着染氟浓度的增加和染氟时间的延长而逐渐下降,染氟6月组大鼠海马及皮质PGC-1α蛋白与血氟含量呈负相关(r=-0. 574、-0. 516,P <0. 05)、与大鼠逗留平台象限时间呈正相关(r=0. 76、0. 60,P <0. 05)、与逃避潜伏期呈负相关(r=-0. 542、-0. 58,P <0. 05)。结论:慢性氟中毒大鼠大脑组织PGC-1α蛋白表达水平降低,可能是氟中毒大鼠空间学习记忆能力降低的机制之一。
Objective: To investigate the changes of peroxisome proliferators-activated receptor γ coactivator(PGC-1α) in the brain of rats with chronic fluorosis and to explore the role of PGC-1α in the pathogenesis of chronic fluorosis and its relationship with learning and memory. Methods: 60 Sprague Dawley(SD) rats were divided randomly into control group(fluoride content of drinking water< 0. 5 mg/L),low fluoride group(fluoride content of drinking water is 5. 0 mg/L). L),high fluoride group(fluoride content of drinking water is 50. 0 mg/L),treatd for 3 and 6 months. The learning and memory ability were determined by Morris water maze test in 3 and 6 months; the fluoride content in serum was detected by Fluoride-ion selective electrode; the protein expression level of PGC-1α in brain tissue was detected by Western blotting; the correlation between PGC-1α protein expression and serum fluoride content and spatial learning and memory indices were also analyzed. Result: The escape latency of rats was significantly longer than that of the control group,the total number of space explorations was significantly reduced,and the time of the quadrant stayed platform was shortened significantly in rats treated for 6 months(P < 0. 05). The blood fluoride content increased with the increase of fluoride concentration and the time of fluoride exposure,and its content in the high fluoride group was significantly higher than that in the control group,especially in the 6-month fluorine-exposure group(P <0. 05). The expression of PGC-1α protein in the hippocampus and cortex of rats treated with fluorine were gradually decreased with the increase of the fluoride concentration and the time of fluoride exposure. The results of correlation analysis showed that there was a negative correlation between PGC-1αprotein in rats brain and blood fluoride content(r =-0. 574,-0. 516,P < 0. 05),and a positive correlation between the expression of PGC-1α protein in rats and resting platform quadrant(r = 0. 76,0. 60,P < 0. 05) and escape latency was negatively correlated(r =-0. 542,-0. 58,P < 0. 05).Conclusion: The decreased expression of PGC-1α protein in brain tissue may be one of the factors influencing the spatial learning and memory ability with chronic fluorosis.

关键词(KeyWords): 氟中毒;过氧化物酶体增殖激活受体γ辅激活因子;学习记忆能力;脑组织;大鼠,Sprague-Dawley
fluorosis;peroxisome proliferator-activated receptor γ coactivator;learning and memory ability;brain tissue;rats,Sprague-Dawley

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81760571);; 贵州省创新计划资助项目[黔教合协同创新中心(2014)06,黔科通(2016)161];; 贵阳市联合基金资助项目[筑科合同(2017)30-3号]

作者(Author): 刘仙红;陈丹;曾晓晓;董阳婷;邓婕;齐晓岚;吴昌学;李毅;宋辉;官志忠;
LIU Xianhong;CHEN Dan;ZENG Xiaoxiao;DONG Yangting;DENG Jie;QI Xiaolan;WU Changxue;LI Yi;SONG Hui;GUAN Zhizhong;Key Laboratory of Endemic and Ethnic Group Disease of Ministry of Education,Guizhou Medical University;Key Laboratory of Molecular Biology of Guizhou Province,Guizhou Medical University;Key Laboratory of Molecular Biology,Guizhou Medical University;Department of Pathophysiology,Affiliated Hospital of Guizhou Medical University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2018.10.007

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