黄盛文,苏晓庆
HUANG Sheng-wen; SU Xiao-qing (Department of Biology;

• 1:贵阳医学院生物学教研室
• 2:贵阳医学院生物学教研室 贵州 贵阳 550004
• 3:贵州 贵阳 550004

摘要(Abstract)：

目的:观察卡地腐霉产生游动孢子所需的环境条件。方法:以卡地腐霉经诱导产生的游动孢子数量为指标,观察不同条件对其产生游动孢子的影响,确定该真菌产生游动孢子所需的最适条件。结果:卡地腐霉产生游动包子的适宜温度范围为10～30℃,最适温度为 24～26％;适宜产孢的蒸馏水pH值范围为5～12,最适pH值为9～11;8 d的培养物产孢量最多;菌块与蒸馏水的比例为1:40时产孢最多;三种光照周期对卡地腐霉的产孢影响不大,紫外线对产孢有抑制作用;NaCl、(NH_4)_2SO_4、蛋白胨和葡萄糖对游动孢子的产生有较强抑制作用,产孢能力对尿素的耐受性极大。结论:本观察所得资料对卡地腐霉的基础研究和应用都有重要价值。
Objective: To investigate the suitable environmental conditions needed by Pythium caro-linianum for sporulation. Methods: The amount of zoospores produced by P. carolinianum under different conditions was chosen as the index to reflect the suitability of the fungus to the conditions. Results: The suitable temperature range for sporulation of the fungus were 10 ℃-30℃, while the optimal temperature range was 24 ℃ - 26 ℃ ; The Suitable pH range was 5 - 12, with an optimum range of 9 - 11; Mycelia of 8 - day old had the highest zoospore yield; The optimal ratio of hyphal pieces to water volume(ml) was 1:40(10:400) for zoospore production; The light cycles did not show influence, but ultraviolet had obvious inhibitory effect on sporulation; Zoospore production was significantly reduced by NaCl, (NH4)2SO4, pepton and glucose at the concentrations of 0.2g/l, 50mg/l, 10mg/l, and 10mg/l respectively, but zoospore could be produced when high concentration of urea solution existed. Conclusions: This research has revealed the requirement of P. carolinianum to environmental conditions for sporulation. Data collected here are useful reference for further basic study and application of P. carolinianum.

关键词(KeyWords)： 灭蚊;害虫防治;,生物学;卡地腐霉;孢子;,真菌;温度;pH值
mosquito control; pest control, biological; Pythium carolinianum; spores, fungal; temperature; pH value

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(批准号30160003)

作者(Authors): 黄盛文,苏晓庆
HUANG Sheng-wen; SU Xiao-qing (Department of Biology;

DOI: 10.19367/j.cnki.1000-2707.2003.02.003

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