卓贤露,姜振东,魏运军,张学渊,袁伟
ZHUO Xianlu,JIANG Zhendong,WEI Yunjun,ZHANG Xueyuan,YUAN Wei(Department of Otolaryngology

• 1:第三军医大学西南医院耳鼻咽喉科
• 2:第三军医大学西南医院耳鼻咽喉科
• 3:第三军医大学西南医院耳鼻咽喉科
• 4:第三军医大学西南医院耳鼻咽喉科

摘要(Abstract)：

目的:探讨Otos基因的表达对碱性成纤维细胞生长因子(bFGF)的调控作用,以揭示Otos基因和bFGF在耳聋病理生理过程中的联系和作用。方法:用含有大鼠Otos基因的腺病毒载体Ad-Otos转染培养的大鼠耳蜗螺旋韧带成纤维细胞,应用RT-PCR技术从转染细胞中检测Otos基因和bFGF基因的表达,用Westernblot方法检测转染细胞中的bFGF蛋白的表达。结果:经过转染Ad-Otos后,成纤维细胞中的bFGF显著上调。结论:Otos基因的表达可能对bFGF表达有影响,并有可能对内耳有保护作用。
Objective:To investigate if there might be any influence of Otos gene expression on the regulation of basic fibroblast growth factor(bFGF),and to reveal the possible relationship and interactions between them and the path-physiological course of epicophosiss.Methods:Cultured spiral ligament fibrocytes of SD rats were transfected with different expression vectors including Ad-Otos which contains Otos gene,AD-GFP containing green flourescence protein gene,and DMEM respectively,and,then,mRNA of Otos and bFGF as well as protein of bFGF were assessed with RT-PCR and Western blot.Results:Compared with those in the control cells,mRNA expression of Otos and mRNA and expressed protein of bFGF in the fibrocytes transfected with Ad-Otos evidently increased.Conclusions:Elevated expression of Otos gene might have an influence on expression of bFGF and have potential protective effects to inner ear.

关键词(KeyWords)： 基因,Otos;成纤维细胞生长因子;耳蜗;大鼠
gene,otos;fibroblast growth factor;cochlea;rats

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 卓贤露,姜振东,魏运军,张学渊,袁伟
ZHUO Xianlu,JIANG Zhendong,WEI Yunjun,ZHANG Xueyuan,YUAN Wei(Department of Otolaryngology

DOI: 10.19367/j.cnki.1000-2707.2007.06.012

参考文献(References)：

• [1]Delprat B,Ruel J,Guitton MJ,et al.Deafness and co-chlear fibrocyte alterations in mice deficient for the inner ear protein otospiralin[J].Mol Cell Biol,2005(2):847-853.
• [2]Delprat B,Boulanger A,Wang J,et al.Downregulation of oto-spiralin,a novel inner ear protein,causes hair cell degenera-tion and deafness[J].J Neurosci,2002(5):1718-1725.
• [3]尹金淑,翟所强,郭维,等.碱性成纤维细胞生长因子/绿色荧光蛋白基因对药物性耳蜗损害的防治作用[J].中华医学杂志,2002(17):1192-1194.
• [4]李洪涛,刘兆华,王锦玲.新生小鼠膜迷路耳蜗外侧壁的组织培养[J].听力学及言语疾病杂志,2006(1):61-63.
• [5]Forge A,Becker D,Casalotti S,et al.Gap junctions in the inner ear:comparison of distribution patterns in differ-ent vertebrates and assessement of connexin composition in mammals[J].J Comp Neurol,2003(2):207-231.
• [6]Caravelli A,Pianese L,Saulino C,et al.Down-regula-tion of otospiralin mRNA in response to acoustic stress in guinea pig[J].Hear Res,2004(1-2):36-40.
• [7]卓贤露,宋宇峰.口腔鳞癌组织中bFGF的表达与血管生成的关系[J].实用口腔医学杂志,2005(3):303-306.
• [8]Wright TJ,Hatch EP,Karabagli H,et al.Expression of mouse fibroblast growth factor and fibroblast growth factor receptor genes during early inner ear development[J].Dev Dyn,2003(2):267-272.
• [9]Low W,Dazert S,Baird A,et al.Basic fibroblast growth factor(FGF-2)protects rat cochlear hair cells in organo-typical culture from aminoglycoside injury[J].J Cell Physiol,1996(3):443-450.
• [10]Sliwinska-Kowalska M,Rzadzinska A,Rajkowska E,et al.Expression of bFGF and NGF and their receptors in chick’s auditory organ following overexposure to noise[J].Hear Res,2005(1-2):93-103.
• [11]Zhai SQ,Wang DJ,Wang JL,et al.Basic fibroblast growth factor protects auditory neurons and hair cells from glutamate neurotoxicity and noise exposure[J].Ac-ta Otolaryngol,2004(2):124-129.

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