柏晓辉,管志敏,潘艳伶
BAI Xiaohui,GUAN Zhimin,PAN Yanling

• 1:贵州医科大学
• 2:贵州医科大学中医科

摘要(Abstract)：

目的:探讨小剂量链脲佐菌素注射液(STZ)联合高能量饮食法诱导糖尿病胃轻瘫(DGP)大鼠模型。方法:将24只大鼠随机均分为正常组和模型组,正常组大鼠予普通饲料喂养,模型组大鼠予高脂高糖喂养,模型组大鼠高脂高糖喂养4周后,予1%STZ溶液腹腔注射3次,剂量分别为20、25、35 mg/kg,第3次注射后4周测大鼠空腹血糖、胃内残留率、血清胃动素(MLT)、胃泌素(GAS),同时取大鼠胃组织,观察胃窦平滑肌细胞。结果:模型组大鼠空腹血糖、胃内残留率、MLT、GAS明显高于正常组,差异具有统计学意义(P<0.01);模型组大鼠胃窦平滑肌细胞形态学改变较正常组大鼠有明显差异。结论:小剂量STZ联合高能量饮食法可成功复制DGP大鼠模型。
Objective: To investigate the establishment of rat model of diabetic gastroparesis( DGP)by low dose of STZ combined with high energy diet. Methods: Twenty-four rats were randomly divided into normal group and model group,12 rats in each group. Rats in normal group were fed with common diet while rats in model group were fed with high energy diet. After four weeks of high energy diet feeding,rats in model group received intraperitoneal injection of low dose of STZ solution three times,20,25,35 mg / kg respectively. Four weeks after the third injection,FBG level,the gastric emptying rate,serum MLT,GAS content of rats were detected. And the gastric tissue of rats was taken out to observe morphological changes of gastric smooth muscle cell. Results: The FBG level,gastric emptying rate,serum MLT and GAS content of rats in model group were significantly higher than their counterparts in normal group( P < 0. 01). The morphological changes of gastric smooth muscle cell in model group were different from those in normal group. Conclusion: Low dose of STZ combined with high energy diet can successfully establish the rat model of DGP.

关键词(KeyWords)： 糖尿病;胃轻瘫;动物,模型;大鼠;链脲佐菌素,小剂量;高脂高糖食物
diabetes mellitus;diabetic gastroparesis;animals model;rat;streptozotocin,small dose;high energy diet

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 柏晓辉,管志敏,潘艳伶
BAI Xiaohui,GUAN Zhimin,PAN Yanling

DOI: 10.19367/j.cnki.1000-2707.2015.10.016

参考文献(References)：

• [1]Gautam A,Baluch A,Kaye A,et al Modern strategies for the anesthetic management of the patient with diabetes[J].Middle East J Anesthesiol,2009(2):187-197.
• [2]许曼音,陆广华.陈明道,等.糖尿病学[M].上海:上海科学技术出版社,2003:50-51.
• [3]陆英杰,连至诚.糖尿病胃轻瘫治疗现状[J].实用糖尿病杂志,2005(6):49-51.
• [4]钟毅,赵自明,陆英杰,等.胃肠舒对糖尿病胃轻瘫大鼠胃窦平滑肌细胞病变的影响[J].中国实验方剂学杂志,2009(9):71-76.
• [5]吴春福,陈多.小鼠胃排空模型的探讨[J].中国药理学通报,1997(3):271-272.
• [6]莫一菲,周健,贾伟平.国际糖尿病联盟2011-2021年全球糖尿病计划解读[J].浙江医学,2013(3):977-979.
• [7]姚冬英,刘菲.糖尿病胃轻瘫发病机制的研究进展[J].国际消化病杂志,2011(1):16-17.
• [8]Chalkley SM,Hettiarachchi M,Chisholm D J,et al.Longterm high-fat feeding leads to severe insulin resistance but not diabetes in Wistar rats[J].Am J Physiol Endocrinol Metab,2002(282):1231-1238.
• [9]Maria So rhede Winzell and Bo Ahren eta l.A Model for Studying Mechanisms and Treatment of Impaired Glucose Tolerance and Type 2 Diabetes[J].Diabetes,2004(supplement3):215-219.
• [10]葛海燕,沈明勤,李娟.中医益气健脾与养阴和胃法对糖尿病胃轻瘫大鼠胃动力和胃血流的作用比较[J].中国实验方剂学杂志,2012(12):238-241.
• [11]赵宏贤,陈霞,杨燕,等.糖尿病胃轻瘫大鼠胃平滑肌细胞超微结构变化[J].现代预防医学,2008(9):1764-1766.
• [12]李丽霞,林国华.电针对糖尿病胃轻瘫大鼠MLT、GAS的影响[J].河南中医学院学报,2006(3):25-27.

文章评论(Comment)：