贵州医科大学学报

2014, v.39;No.169(04) 490-493

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UFLC-UV法同时测定黔产丹参中4个水溶性成分的含量
UFLC-UV Simultaneous Determination of Four Water-soluble Compounds in Salvia miltiorrhiza from Guizhou

王秋平;郑林;陈思颖;黄勇;兰燕宇;
WANG Qiuping;ZHENG Lin;CHEN Siying;HUANG Yong;LAN Yanyu;People's Hospital of Guizhou Province;School of Pharmacy,Guiyang Medical University;

摘要(Abstract):

目的:建立超快速液相色谱(UFLC)的方法同时测定丹参成分丹参素、迷迭香酸、丹酚酸B、丹酚酸A。方法:采用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.05%磷酸为流动相进行梯度洗脱,流速1 mL/min,检测波长220 nm,柱温40℃,检测贵州4个不同产地的8批丹参成分丹参素、迷迭香酸、丹酚酸B、丹酚酸A。结果:4种被测成分的线性关系良好(r>0.999),精密度RSD 0.49%~1.67%,重复性RSD1.27%~2.17%,稳定性RSD 1.53%~2.85%,平均加样回收率在96.80%~103.08%,RSD 1.88%~2.79%(n=6),8批黔产丹参药材中丹参素、迷迭香酸、丹酚酸B、丹酚酸A的平均含量分别为2.061、3.559、44.223及1.426 mg/g。结论:UFLC法用于检测丹参药材中丹参素、迷迭香酸、丹酚酸B、丹酚酸A,具有简便、快速、准确的优点,能有效控制丹参药材的质量。
Objective: To establish a UFLC-UV method for determination of four water-soluble compounds in Salvia miltiorrhiza of Guizhou. Methods: Chromatogaphic analysis was performed on a Diamonsil C18column(4. 6 mm × 250 mm,5 μm) with acetonitrile-0. 05% phosphoric acid as mobile phase in gradient elution mode. The flow rate was 1 mL /min with the detection wavelength of 220 nm.The column temperature was set at 40 ℃. 8 samples of 4 different producing areas from Guizhou were analyzed. Result: All the four water-soluble compounds showed good linearity( r > 0. 999) in the range of tests concentrations. The RSD of the precision,reproducibility and stability tests were 0. 49%~ 1. 67%,1. 27% ~ 2. 17%,1. 53% ~ 2. 85% respectively; the average recoveries of the method were in the range of 96. 80% ~ 103. 08%,RSD 1. 88% ~ 2. 79%(n = 6). The average contents of four water-soluble compounds in Salvia miltiorrhiza of Guizhou were 2. 061,3. 559,44. 223,1. 426 mg /g respectively. Conclusions: The method is simple,rapid and accurate,and can be applied for the quality control of Salvia miltiorrhiza from Guizhou.

关键词(KeyWords): 色谱法,液相;贵州;丹参;水溶性多酚
chromatography,liquid;Guizhou;Salvia miltiorrhiza;water-soluble compounds

Abstract:

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基金项目(Foundation): 贵州省科技重大专项项目[黔科合重大专项字(2011)6019号];; 贵阳市科技计划项目[筑科合同(2011401)社6-2号];; 贵州省中药现代化科技产业研究开发专项[黔科合重G字(2013)4001]

作者(Author): 王秋平;郑林;陈思颖;黄勇;兰燕宇;
WANG Qiuping;ZHENG Lin;CHEN Siying;HUANG Yong;LAN Yanyu;People's Hospital of Guizhou Province;School of Pharmacy,Guiyang Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2014.04.012

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