贵州医科大学学报

2016, v.41;No.193(10) 1153-1158

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LIN28 mRNA在肝细胞癌中的表达及其临床意义
The Expression and Clinical Value of LIN28 in Hepatocellular Carcinoma

游经宏;元云飞;周凯;
YOU Jinghong;YUAN Yunfei;ZHOU Kai;Department of General Surgery,Jianli County People's Hospital;Department of Hepatology,Sun yat-sen University Cancer Hospital;Department of Surgery,Jiangxi province People's Hospital;

摘要(Abstract):

目的:研究肝细胞癌LIN28 mRNA表达水平与临床病理因素和预后的关系。方法:用半定量RTPCR检测人肝癌细胞系Hep-G2、MHCC-97H、SMMC-7721、BEL-7402细胞、永生化肝细胞系L-O2肝细胞、92例肝细胞癌组织和相应的癌旁肝组织及20例正常无肝硬化肝组织中的LIN28 mRNA表达水平,比较不同临床病理因素和预后(1年、3年、5年总体生存率和无瘤生存率)的肝细胞癌组织的LIN28 mRNA阳性率,分析影响肝癌切除术后患者总生存率和无瘤生存率的危险因素。结果:LIN28 mRNA在人肝癌细胞Hep-G2、MHCC-97H、SMMC-7721及BEL-7402中均表达,在永生化肝细胞系L-O2中无表达;LIN28 mRNA在肝癌组织中的表达率为70.7%,癌旁肝组织为37.0%(P<0.001),在正常无肝硬化肝组织中无表达;肝癌组织中LIN28 mRNA的表达与血管侵犯、病理分级和TNM分期有关(P<0.05);LIN28 mRNA阳性患者具有较短的总生存期及无瘤生存期,肝癌切除术后患者总生存时间与肿瘤大小、血管侵犯、病理分级及TNM分期等因素有关(P<0.05),无瘤生存时间则与肿瘤大小、肿瘤数目、血管侵犯、病理分级及TNM分期相关(P<0.05);LIN28 mRNA的表达不是总生存率及无瘤生存率的独立影响因素。结论:LIN28 mRNA在肝癌细胞系及肝癌组织中高表达,可能在肝癌发生发展中发挥重要作用,可以作为肝癌患者预后预测的一个参考指标。
Objective: To explore the expression of LIN28 mRNA in HCC,and its correlations with clinicopathologic features and prognosis. Methods: LIN28 mRNA expression was detected in four liver cancer cell lines( Hep-G2,MHCC97,SMMC-7721 and BEL-7402),one immortalized liver cell line L-O2,tumor tissues with matched non-neoplastic liver tissues in 92 HCC patients and normal liver tissues of 20 cases using semi-quantitative reverse transcription-polymerase chain reaction( RT-PCR).The correlations between LIN28 mRNA and clinicopathologic features and prognosis of HCC were analyzed( overall survival rate and disease-free survival rate of 1 year,3 years,5 years),then carrying out analysis on risk factors of overall survival rate and disease-free survival rate after resection of hepatocellular carcinoma. Result: LIN28 mRNA was detected in Hep-G2,MHCC-97 H,SMMC-7721 and BEL-7402,but absent in L-O2. The positive rate of LIN28 mRNA expression was significantly higher in HCC tissues than in non-neoplastic liver tissues( 70. 7% vs. 37. 0%,P < 0. 001). LIN28 mRNA expression was not detected in the normal liver tissues. LIN28 mRNA expression was correlated with the vascular invasion,differentiation and TNM stage( P < 0. 05). Kaplan-Meier survival curves showed that patients with positive expression of LIN28 mRNA had lower overall survival and diseasefree survival rates; LIN28 mRNA expression was not the independent influential factor of overall survival and disease-free survival. Conclusion: LIN28 mRNA,which is highly expressed in a subset of liver cancer cell lines and HCC tissues,may be involved in hepatocarcinogenesis. LIN28 mRNA may be a valuable biomarker for assessing prognosis of HCC.

关键词(KeyWords): 癌,肝细胞;肿瘤干细胞;RNA结合蛋白;肿瘤分期;预后
carcinoma;hepatocellular;tumor stem cell;RNA binding protein;noeoplasm staging;prognosis

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基金项目(Foundation): 国家自然科学基金项目(No.30972916)

作者(Author): 游经宏;元云飞;周凯;
YOU Jinghong;YUAN Yunfei;ZHOU Kai;Department of General Surgery,Jianli County People's Hospital;Department of Hepatology,Sun yat-sen University Cancer Hospital;Department of Surgery,Jiangxi province People's Hospital;

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DOI: 10.19367/j.cnki.1000-2707.2016.10.008

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