张可训,张建鹏,兰亚娟
ZHANG Kexun,ZHANG Jianpeng,LAN Yajuan

• 1:三原县医院

摘要(Abstract)：

目的:探讨B、T淋巴细胞衰减因子(BTLA)、血清白介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)及部分血小板指标在膝关节炎(OA)新西兰家兔外周血中的变化。方法:30只新西兰家兔均分为模型组、治疗组和假手术组,切断模型组和治疗组新西兰家兔前交叉韧带、剪除内侧半月板、建立兔膝OA模型,假手术组仅打开关节腔;手术结束3周时流式细胞技术检测3组大鼠外周血CD3~+BTLA~+T细胞、CD4~+BTLA~+T细胞、CD8~+BTLA~+T细胞及调节性T细胞(Treg),ELISA法检测血清IL-1、TNF-α、IL-10水平,全自动生化分析仪检测血小板计数(PLT)、血小板压积(PCT)、血小板分布宽度(PDW)及血小板平均体积(MPG)。结果:造模成功后3周时模型组家兔外周血IL-1、TNF-α、IL-10水平高于另外两组(P<0.05),CD3~+BTLA~+T细胞、CD4~+BTLA~+T细胞、CD8~+BTLA~+T细胞及Treg百分比均明显低于另外两组(P<0.05),PLT及PCT均明显低于另外两组(P<0.05);模型组家兔外周血PLT与CD3~+BTLA~+T细胞、CD4~+BTLA~+T细胞及Treg水平成正相关;PCT、PDW与CD8~+BTLA~+T细胞水平成正相关,MPV与CD4~+BTLA~+T细胞水平成正相关(P<0.05)。结论:BTLA阳性T淋巴细胞水平、IL-1、TNF-α、IL-10以及部分血小板参数可能参与了OA的发生发展。
Objective: To explore the changes of B-lymphocyte and T-lymphocyte attenuation factors( BTLA),serum interleukin-1( IL-1),tumor necrosis factor-α( TNF-α),and interleukin-10( IL-10)and some thrombocyte parameters in New zealand rabbit knee osteoarthritis( OA) model. Methods:Thirty New Zealand white rabbits were randomly divided into three groups: sham operation( group S),treatment group( group T),and model group( group M). Rabbit knee OA model was established by cut anterior cruciate ligament and medial meniscus in groups T and M,while rabbits in group S only received operation of opening knee articular cavity. In 3 months after the operations,CD3~+BTLA~+T cells,CD4~+BTLA~+T cells,CD8~+BTLA~+T cells and Treg cells were detected by flow cytometry,levels of IL-1,TNF-α and IL-10 were measured by enzyme-linked immunosorbent assay( ELISA),and platelet count( PLT),platelet cubic measure( PCT),platelet distribution width( PDW),and platelet mean volume( MPV) were measured by automatic biochemical analyzer. Results: In 3 weeks after the model was successfully established,the levels of IL-1,TNF-α,and IL-10 in group M were higher than those in groups S and T( P < 0. 05),while cell counts of CD3~+BTLA~+T,CD4~+BTLA~+T,CD8~+BTLA~+T,and Treg percentage in group M were significantly lower than those in groups S and T( P <0. 05). PLT and PCT of group M were lower than those of groups S and T( P < 0. 05). In peripheral blood of rabbits in group M,PLT was positively correlated with CD3~+BTLA~+T cells,CD4~+BTLA~+T cells and Treg level( P < 0. 05),PCT and PDW were positively correlated with CD8~+BTLA~+T cells( P < 0. 05),and MPV was positively correlated with CD4~+BTLA~+T cells( P < 0. 05). Conclusion:BTLA,T-lymphocyte levels,IL-1,TNF-α,IL-10 and some platelet parameters might participate the occur and development of OA.

关键词(KeyWords)： 骨关节炎;B、T淋巴细胞衰减因子;白介素-1;肿瘤坏死因子-α;白介素-10;血小板;兔
osteoarthritis;B,T-lymphocyte attenuation factors;interleukin-1;tumor necrosis factor-α;interleukin-10;platelets;rabbits

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张可训,张建鹏,兰亚娟
ZHANG Kexun,ZHANG Jianpeng,LAN Yajuan

DOI: 10.19367/j.cnki.1000-2707.2018.07.011

参考文献(References)：

• [1]SCANZELLO C R,GOLDRING S R.The role of synovitis in osteoarthritis pathogenesis[J].Bone,2012,51(2):249-257.
• [2]DE LANGE-BROKAAR B J E,IOAN-FACSINAY A,VAN OSCH G,et al.Synovial inflammation,immune cells and their cytokines in osteoarthritis:A review[J].Osteoarthritis and Cartilage,2012,20(12):1484-1499.
• [3]MABEY T,HONSAWEK S.Cytokines as biochemical markers for knee osteoarthritis[J].World J Orthop,2015,6(1):95-105.
• [4]LAMPROPOULOU-ADAMIDOU K,LELOVAS P,KARADIMAS E V,et al.Useful animal models for the research of osteoarthritis[J].European Journal of Orthopaedic Surgery&Traumatology,2014,24(3):263-271.
• [5]ZHANG J,SONG L,LIU G,et al.Risk factors for and prevalence of knee osteoarthritis in the rural areas of Shanxi Province,North China:A COPCORD study[J].Rheumatology International,2013,33(11):2783-2788.
• [6]COOPER C,JAVAID M K,Arden N.Epidemiology of osteoarthritis[M].Atlas of Osteoarthritis,2014(1):21-36.
• [7]JOHNSON V L,HUNTER D J.The epidemiology of osteoarthritis[J].Best practice&research Clinical rheumatology,2014,28(1):5-15.
• [8]TANG X,WANG S,ZHAN S,et al.The Prevalence of symptomatic knee osteoarthritis in China:Results from the China health and retirement longitudinal study[J].Arthritis&Rheumatology,2016,68(3):648-653.
• [9]LURATI A,LARIA A,MAZZOCCHI D,et al.Effects of hyaluronic acid(HA)viscosupplementation on peripheral Th cells in knee and hip osteoarthritis[J].Osteoarthritis and Cartilage,2015,23(1):88-93.
• [10]HASEEB A,HAQQI T M.Immunopathogenesis of osteoarthritis[J].Clinical Immunology,2013,146(3):185-196.
• [11]LUBBERTS E.Role of T lymphocytes in the development of rheumatoid arthritis:Implications for treatment[J].Current Pharmaceutical Design,2015,21(2):142-146.
• [12]ATUKORALA I,KWOH C K,Guermazi A,et al.Synovitis in knee osteoarthritis:A precursor of disease[J].Annals of the Rheumatic Diseases,2016,75(2):390-395.
• [13]HOUARD X,GOLDRING M B,Berenbaum F.Homeostatic mechanisms in articular cartilage and role of inflammation in osteoarthritis[J].Current Rheumatology Reports,2013,15(11):1-10.
• [14]WOJDASIEWICZ P,PONIATOWSKIA,SZUKIEWICZ D.The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of osteoarthritis[J].Mediators of Inflammation,2014,2014:561459.
• [15]KRIEG C,HAN P,STONE R,et al.Functional analysis of B and T lymphocyte attenuator engagement on CD4+and CD8+T cells[J].The Journal of Immunology,2005,175(10):6420-6427.
• [16]SUNDMAN E A,COLE B J,KARAS V,et al.The antiinflammatory and matrix restorative mechanisms of platelet-rich plasma in osteoarthritis[J].The American Journal of Sports Medicine,2014,42(1):35-41.

文章评论(Comment)：