袁桃花;李欣芯;陈丽亚;曹胜宇;孙见飞;刘华;吴昌学;吴宁;
YUAN Taohua;LI Xinxin;CHEN Liya;CAO Shengyu;SUN Jianfei;LIU Hua;WU Changxue;WU Ning;School of Basic Medical Science, Guizhou Medical University;Key Laboratory of Molecular Biology, Guizhou Medical University;

• 1:贵州医科大学基础医学院
• 2:贵州医科大学
• 3:贵州医科大学分子生物学重点实验室

摘要(Abstract)：

目的:探讨苗药验方"四大血"(SX)对牛Ⅱ型胶原蛋白诱导关节炎(CIA)大鼠白细胞介素17(IL-17)及血管内皮生长因子(VEGF)表达水平的影响。方法:42只SD大鼠分为空白对照组(NG组,n=7)及造模组(n=35),除NG组外,所有大鼠用牛Ⅱ型胶原与等量不完全弗氏佐剂混合乳化剂于尾根部皮下注射致炎、复制CIA动物模型,造模第14天将造模组大鼠均分为模型对照组(Mod组),SX高、中、低药物治疗组(分别给予SX 40、20及10 g/kg灌胃,21 d,n=7)及阳性药物对照组(GTW组,给予雷公藤多甙片40 g/kg灌胃,21 d,n=7);于造模第14天、给药治疗第7、14及21天时检测足肿胀度(ER);给药第21天时,观察大鼠膝关节滑膜组织病理改变,采用实时荧光定量PCR(qRT-PCR)法测定大鼠膝关节滑膜组织VEGF mRNA的表达,ELISA法检测测大鼠血清IL-17及VEGF水平。结果:与Mod组相比,SX 40、20及10 g/kg组、GTW组大鼠足关节ER减轻,关节滑膜组织炎性细胞浸润明显下降,纤维组织增生及水肿程度减轻;治疗第21天时,与Mod组比较,SX 40、20及10 g/kg组大鼠膝关节滑膜组织VEGF mRNA水平、血清VEGF含量均显著降低(P<0.01),SX 40、20 g/kg组血清IL-17含量显著降低(P<0.01)。结论:SX能有效缓解CIA病情,其机制可能与降低IL-17和VEGF含量有关。
Objective: To study the effect of "Sidaxue" of Miao medicine prescription on the expression levels of IL-17 and VEGF in CIA rats. Methods: 42 SD rats were divided into blank control group(NG group, n=7) and module group(n=35). In addition to the NG group, all the rats were subcutaneously injected with bovine type II collagen and equivalent Freund's adjuvant to induce inflammation and replicate CIA animal model. On the 14 th day, the rats in the model group were divided into model control group(Mod group), SX high, medium and low dose treatment group(respectively give SX 40, 20 and 10 g/kg to fill the stomach, 21 d, n=7) and positive drug control(GTW group, give GTW 40 g/kg to fill the stomach, 21 d, n=7). The degree of swelling was detected on the 14 th day of modeling and the 7 th, 14 th and 21 day of administration. After 21 th day of administration, synovial tissue of the knee joint was taken to prepare pathological tissue sections. The expression of VEGF mRNA in rat synovial tissue was detected by qRT-PCR and the expression levels of IL-17 and VEGF in rat serum were detected by ELISA. Results: Compared with the Mod group, the swelling of the rat foot joints in the SX 40, 20 and 10 g/kg groups and the GTW group was alleviated; the infiltration of inflammatory cells was significantly decreased; the fibrous tissue hyperplasia and edema extent were alleviated. Compared with the Mod group on 21 th day, mRNA levels of VEGF in SX 40, 20 and 10 g/kg serum were significantly down-regulated(P<0.01), and IL-17 levels in SX 40 g/kg and 20 g/kg groups were decreased(P<0.01). Conclusions: SX can effectively alleviate the condition of CIA by reducing the expression levels of IL-17 and VEGF.

关键词(KeyWords)： 类风湿性关节炎;白细胞介素17;血管内皮生长因子;苗药验方“四大血”;牛Ⅱ型胶原蛋白诱导关节炎大鼠模型;大鼠,Sprague-Dawley
rheumatoid arthritis;interleukin 17;vascular endothelial growth factor;Miao medicine prescription "Sidaxue";bovine type Ⅱ collagen induced arthritis rat model;rats,Sprague-Dawley

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技合作计划项目[黔科合LH字(2015)7330];; 2014年贵州省卫生计生委科学技术基金项目;; 贵州省2018年大学生创新创业训练计划项目(2018520333、2018520352、2018520353)

作者(Authors): 袁桃花;李欣芯;陈丽亚;曹胜宇;孙见飞;刘华;吴昌学;吴宁;
YUAN Taohua;LI Xinxin;CHEN Liya;CAO Shengyu;SUN Jianfei;LIU Hua;WU Changxue;WU Ning;School of Basic Medical Science, Guizhou Medical University;Key Laboratory of Molecular Biology, Guizhou Medical University;

DOI: 10.19367/j.cnki.1000-2707.2019.06.009

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